, Volume 64, Issue 11–12, pp 695–699 | Cite as

Preparative Droplet Counter-Current Chromatography for the Separation of the New Nor-Seco-Triterpene and Pentacyclic Triterpenoids from Qualea Parviflora

  • A. L. M. NasserEmail author
  • L. P. Mazzolin
  • C. A. Hiruma-Lima
  • L. S. Santos
  • M. N. Eberlin
  • A. R. Monteiro de Souza Brito
  • W. Vilegas


The methanolic extract of the bark of the medicinal plant Qualea parviflora (Vochysiaceae) contains new nor-seco-triterpene and pentacyclic triterpenoids. They were separated in a preparative scale using droplet counter-current chromatography. The optimum solvent used was composed of a mixture of CHCl3/MeOH/H2O (43:37:20, v/v) in the descending mode and led to a successful separation of the new compound 28-nor-17, 22-seco-2α, 3β, 19, 22, 23-pentahydroxy-Δ12-oleanane, besides the known triterpenoids bellericagenin B, bellericaside B and arjunglucoside I . Identification was performed by ESI-MS, 1H NMR and 13C NMR analyses.


Thin Layer chromatography Droplet counter-current chromatography Nor-seco-triterpene and pentacyclic triterpenoids Qualea parviflora bark 



The authors thank Dr. Nivaldo Boralle from the Instituto de Química de Araraquara –SP- for recording the NMR spectra, to Roberto Sigfrido Gallegos Olea from the Universidade Federal do Maranhão for the helpful discussions, to Fundação de Amparo à Pesquisa do Estado de São Paulo, for supporting this work and to a fellowship to ALMN, and to the Conselho Nacional de Desenvolvimento Científico e Tecnológico, for grants to ARMSB and WV.


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  • A. L. M. Nasser
    • 1
    Email author
  • L. P. Mazzolin
    • 2
  • C. A. Hiruma-Lima
    • 2
  • L. S. Santos
    • 3
  • M. N. Eberlin
    • 3
  • A. R. Monteiro de Souza Brito
    • 4
  • W. Vilegas
    • 1
  1. 1.Instituto de Química de AraraquaraUniversidade Estadual PaulistaAraraquaraBrazil
  2. 2.Departamento de Farmacologia, Instituto de BiociênciasUniversidade Estadual PaulistaBotucatuBrazil
  3. 3.Instituto de QuímicaUniversidade Estadual de CampinasCampinasBrazil
  4. 4.Departamento de Fisiologia e Biofísica, Instituto de BiologiaUniversidade Estadual de CampinasCampinasBrazil

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