, Volume 64, Issue 5–6, pp 307–311 | Cite as

Selective Determination of Sulfonamide Residues in Honey by SPE-RP-LC with UV Detection

Short Communication


Solid-phase extraction was used to isolate sulfacetamide, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethoxypyridazine and sulfamethoxazole from honey. The optimized procedure used polymeric Abselut Nexus cartridges and the sulfonamides were separated, in the isocratic mode, on an Inertsil ODS-3 (250 × 4 mm I.D., 5 μm) column, using methanol-0.05 M acetate buffer (pH 3.6) (20:80 v/v) with 1% (v/v) of acetic acid, UV detection at 263 nm and a flow-rate of 1 mL min−1. Caffeine was used as internal standard. Average recoveries of the analytes from spiked honey ranged from 80 to 117% and the detection limits based on a spiked honey extract were 20–25 μg kg−1.


Column liquid chromatography Solid-phase extraction Sulfonamides Honey 


  1. 1.
    Maudens KE, Zhang GF, Lambert WE (2004) J Chromatogr A 1047:85–92CrossRefGoogle Scholar
  2. 2.
  3. 3.
    Harle DG, Baldo BA, Wells JV (1988) Mol Immunol 25:1347–1354CrossRefGoogle Scholar
  4. 4.
    Commission of the European Communities, Council Regulation EEC 2377/90 Off. J. L 224 (1990) 1Google Scholar
  5. 5.
    Barry CP, MacEachern GM (1983) J Assoc Off Anal Chem 66:4–7Google Scholar
  6. 6.
    Schwartz DP, Sherma J (1986) J Assoc Off Anal Chem 69:72–76Google Scholar
  7. 7.
    Sherma J, Bretschneider W, Dittamo M, Dibiase N, Huh D, Schwartz DP (1989) J Chromatogr 463:229–233CrossRefGoogle Scholar
  8. 8.
    Sheth HB, Yaylayan VA, Low NH, Stiles ME, Sporns P (1990) J Agric Food Chem 38:1125–1130CrossRefGoogle Scholar
  9. 9.
    Thomson CA, Sporns P (1995) J Food Sci 60:409–415CrossRefGoogle Scholar
  10. 10.
    Heering W, Usleber E, Dietrich R, Märtlbauer E (1998) Analyst 123:2759–2762CrossRefGoogle Scholar
  11. 11.
    Neidert E, Baraniak Z, Sauve A (1986) J Assoc Off Anal Chem 69:641–645Google Scholar
  12. 12.
    Belliardo F (1981) J Apic Res 20:44–48Google Scholar
  13. 13.
    Diaz TG, Cabanillas AG, Salinas F (1990) Anal Lett 23:607–616Google Scholar
  14. 14.
    Horie M, Saito K, Nose N, Nakazawa H (1992) J AOAC Int 75:786–789Google Scholar
  15. 15.
    Vinas P, Erroz CL, Canals AH, Cordoba MH (1995) Chromatographia 40:382–386CrossRefGoogle Scholar
  16. 16.
    Szymanski A (2000) Chem Anal 45:521–528Google Scholar
  17. 17.
    Caballero RD, Torres-Lapasio JR, Baeza-Baeza JJ, Garcia-Alvarez-Coque MC (2001) J Liq Chromatogr Relat Technol 24:117–131CrossRefGoogle Scholar
  18. 18.
    Posyniak A, Sniegocki T, Zmudzki J (2002) Bull Vet Inst Pulawy 46:111–117Google Scholar
  19. 19.
    Kaufmann A, Roth S, Ryser B, Widmer M, Guggisberg D (2002) J AOAC Int 85:853–860Google Scholar
  20. 20.
    Verzegnassi L, Savoy-Perroud MC, Stadler RH (2002) J Chromatogr A 977:77–87CrossRefGoogle Scholar
  21. 21.
    Martel AC, Zeggane S (2003) J Liq Chromatogr Relat Technol 26:953–961CrossRefGoogle Scholar
  22. 22.
    Pang GF, Cao YZ, Fann CL, Zhang JJ, Li XM, Jia GO (2003) Anal Bioanal Chem 376:534–541CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.Laboratory of Analytical Chemistry, Department of ChemistryAristotle University of ThessalonikiThessalonikiGreece

Personalised recommendations