Chromatographia

, Volume 62, Supplement 13, pp s7–s17 | Cite as

Searching for Robust HPLC Methods – Csaba Horváth and the Solvophobic Theory

Article
First Online:
Received:
Revised:
Accepted:

Abstract

This paper is written in remembrance of the work of Csaba Horváth on Reversed Phase Chromatography, (RPC) and the fundamental theory of the mechanism of retention on non-polar stationary phases, the ``Solvophobic Theory''. The paper discusses some steps in the development of this important theory and examines its consequences in developing robust methods for routine RPC. Reliable product quality requires the understanding of selectivity changes, which in RPC govern the development of robust and reliable methods involving continuous changes of liquid chromatographic parameters in aqueous eluents. The application of RPC is still growing in scientific research and in pharmaceutical and chemical production. The impact of the Solvophobic Theory in life sciences has been enormous but it was only a part of Horváth's scientific work. RPC is today one of the most popular, most widely used tools in analytical chemistry and will remain so for many years due to its stability and to its robustness.

Keywords

Csaba Horváth Column liquid chromatography Reversed phase chromatography Solvophobic theory retention modeling Continuous changes of selectivity 
This is a preview of subscription content, log in to check access.

References

  1. Horváth Cs, Melander W, Molnár I (1976) J Chromatogr 125:129–156Google Scholar
  2. Horváth Cs, Melander W, Molnár I (1977) Analyt Chem 49:142–154Google Scholar
  3. Horváth Cs, Melander W, Molnár I, Molnár P (1977) Analyt Chem 49:2295-2305Google Scholar
  4. Csaba Horváth, private communication Aug. 1975Google Scholar
  5. Benedek K, Dong S, Karger BL (1984) J Chromatogr 317:227–243CrossRefGoogle Scholar
  6. Scott CD (1968) Clin Chem 14:521–528Google Scholar
  7. Molnár I, Horváth Cs (1977) J Chromatogr 143:391–400Google Scholar
  8. Stout RW, Michelot RJ, Molnár I, Horváth Cs, Coward JK (1976) Analyt Biochem 76:330–341Google Scholar
  9. Molnár I, Horváth C (1978) J Chromatogr 145:371–381Google Scholar
  10. Molnár I, Horváth Cs (1976) Clin Chem 22:1497–1502Google Scholar
  11. Molnár I, Horváth Cs, Jatlow P (1978) Chromatographia 11:260–265Google Scholar
  12. Molnár I, Horváth Cs (1977) J Chromatogr 442:623–640Google Scholar
  13. Molnár I (2002) J Chromatogr A 965:175–194Google Scholar
  14. Jacobson J, El Rassi Z, Horváth Cs (1983) In: Molnár I (ed) Practical Aspects of Modern HPLC. Verlag Walter de Gruyter & Co., Berlin, New York, pp. 15–23Google Scholar
  15. El Rassi Z, Horváth Cs (1983) In: Molnár I (ed) Practical Aspects of Modern HPLC. Verlag Walter de Gruyter & Co., Berlin, New York, pp. 1–14Google Scholar
  16. Vailaya A, Horváth Cs (1998) J Chromatogr A 829:1–27CrossRefGoogle Scholar
  17. Canals I, Valkó K, Bosch E, Hill AP, Rosés N (2001) Anal Chem 73:4937–4945CrossRefGoogle Scholar
  18. Jupille TH, Dolan JW, Snyder LR, Molnár I (2002) J Chromatogr 948:35–41CrossRefGoogle Scholar
  19. Chloupek RC, Hancock WS, Snyder LR (1992) J Chromatogr 594:65–70CrossRefGoogle Scholar
  20. Lewis JA, Lommen DC, Raddatz WD, Dolan JW, Snyder LR, Molnár I (1992) J Chromatogr 592:183–195Google Scholar
  21. Lewis JA, Lommen DC, Raddatz WD, Dolan JW, Snyder LR, Molnár I (1992) J Chromatogr 592:197–208Google Scholar
  22. Bilke HW, Molnár I, Gernet C (1996) J Chromatog A 729:189–195CrossRefGoogle Scholar
  23. Schmidt AH, Molnár I (2002) J Chromatogr 948, 51–59Google Scholar
  24. Rieger HJ, Molnár I (2002) J Chromatogr 948:43–49CrossRefGoogle Scholar
  25. Snyder LR (1978) J Chromatogr Sci 16:223–230Google Scholar
  26. Glajch JL, Kirland JJ, Snyder LR (1982) J Chromatogr 238:269–277CrossRefGoogle Scholar
  27. Nyiredy Sz, Dallenbach-Toelke K, Sticher O (1989) J Liq Chromatogr 12:95–116Google Scholar
  28. Berridge JC (1985) Techniques for the Automated Optimization of HPLC Separations. John Wiley & SonsGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  1. 1.Institut für angewandte ChromatographieBerlinGermany

Personalised recommendations