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Chromatographia

, Volume 61, Issue 9–10, pp 515–518 | Cite as

Quantitative Determination of Lignan Constituents from Schisandra chinensis by Liquid Chromatography

  • B. Avula
  • Y-W. Choi
  • P. V. Srinivas
  • I. A. KhanEmail author
Article

Abstract

The fruits of Schisandra chinensis (Schisandraceae/Magnoliaceae) are a traditional oriental medicine possessing diverse biological activities. A simple and specific analytical method for the quantitative determination of eight lignan constituents from the methanolic extract of the fruits of Schisandra chinensis was developed. The lignan constituents present in the fruits of Schisandra chinensis were separated with an acetonitrile-water-reagent alcohol gradient at a flow rate of 1.0 mL per minute. The HPLC separation was performed on a Phenomenex Luna C18 (2) (150 × 4.6 mm I.D., particle size 5 μm) reversed phase column with detection at 215 nm. The limit of detection was in the range from 0.2 to1.5 μg mL−1. The relative standard deviation (RSD) values for the determination of lignan constituents in fruits of Schisandra chinensis were less than 2.0%. The method was successfully used to analyze different products available in the market containing Schisandra chinensis and also to study the percentage compositions of various lignans present in Schisandra chinensis procured from different regions in S. Korea.

Keywords

Column liquid chromatography Lignans Dietary supplements Schisandra chinensis 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  • B. Avula
    • 1
  • Y-W. Choi
    • 1
    • 3
  • P. V. Srinivas
    • 1
  • I. A. Khan
    • 1
    • 2
    Email author
  1. 1.National Center for Natural Products ResearchResearch Institute of Pharmaceutical Sciences 
  2. 2.Department of Pharmacognosy, School of PharmacyThe University of MississippiUSA
  3. 3.Department of HorticultureMiryang National UniversityMiryangKorea

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