Chromatographia

, Volume 61, Issue 9–10, pp 443–446 | Cite as

Quantitative Determination of Eugenol from Cinnamomum tamala Nees and Eberm. Leaf Powder and Polyherbal Formulation Using Reverse Phase Liquid Chromatography

  • V. V. Dighe
  • A. A. Gursale
  • R. T. Sane
  • S. Menon
  • P. H. Patel
Article

Abstract

A simple, rapid and precise reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of eugenol from the extract of dried powder of Cinnamomum tamala leaves and its polyherbal formulation. Chromatographic analysis was carried out on Zorbax C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase of mixture of water, acetonitrile and methanol in the volume ratio of 50:40:10, at a flow rate of 1.0 mL min-1. Quantitation was performed using a UV-visible detector at 210 nm. Good linearity was obtained over the ranges of 0.20–3.0 μg mL-1 for eugenol.

Keywords

Column liquid chromatography HPLC Eugenol Cinnamomum tamala Nees Eberm leaf powder 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  • V. V. Dighe
    • 1
  • A. A. Gursale
    • 2
  • R. T. Sane
    • 1
    • 2
  • S. Menon
    • 2
  • P. H. Patel
    • 2
  1. 1.Department of ChemistryMumbaiIndia
  2. 2.Therapeutic Drug Monitoring LaboratoryMumbaiIndia

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