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Chromatographia

, Volume 59, Supplement 1, pp S3–S11 | Cite as

Monitoring Anabolic Steroids in Meat-Producing Animals. Review of Current Hyphenated Mass Spectrometric Techniques

  • B. Le BizecEmail author
  • P. Marchand
  • D. Maume
  • F. Monteau
  • F. André
Article

Abstract

Monitoring anabolic steroids in meat-producing animals is a challenging task. It implies very specific and sensitive analytical methods able to detect and identify sub-μg kg−1 residue levels in complex biological matrices such as meat, urine, or hair. Gas and liquid chromatography coupled to mass spectrometry are the most efficient means of achieving these objectives. In this paper we review how developments in mass spectrometry have been rapidly applied to this problem, how efficient analysis of anabolic steroids in urine, edible tissue, and hair has been achieved, and, later, how measurement of conjugate steroids and determination of the origin of natural steroid hormones has been achieved. The performance characteristics of different mass spectrometers (quadrupole, ion-trap, electromagnetic, isotope-ratio, tandem, and hybrid instruments), the efficiency of different acquisition techniques (LR-SIM, HR-SIM, MRM), and, finally, sample introduction (gas chromatography and liquid chromatography, with discussion of alternative interfaces) are discussed, with numerous applications.

Keywords

Column liquid chromatography mass spectrometry Gas chromatography mass spectrometry Isotope-ratio mass spectrometry Anabolic steroids 

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Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2004

Authors and Affiliations

  • B. Le Bizec
    • 1
    Email author
  • P. Marchand
    • 1
  • D. Maume
    • 1
  • F. Monteau
    • 1
  • F. André
    • 1
  1. 1.Laberca, Ecole Nationale VétérinaireAtlanpôle La ChantrerieNantes Cedex 03France

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