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Chromatographia

, Volume 58, Issue 11–12, pp 701–706 | Cite as

Rapid Purification of Molecular Chaperonins by Flowthrough Chromatography with Customized Biporous Anion-Exchanger

  • Jianlin Chen
  • Shu Bai
  • Yan SunEmail author
Article
  • 34 Downloads

Summary

A rigid spherical biporous matrix was prepared by radical suspension polymerization. Functionalized with diethylamine, a biporous ion-exchange resin (IER-B) was obtained. The properties of IER-B were compared with the microporous ion exchange resin (IER-M) in pore size distributions and specific surface areas. Batch adsorption showed that IER-B and IER-M had comparable static adsorption capacities for BSA, i.e., 55.6 and 63.8 mg · g-1 wet resin, respectively. The dynamic capacity of IER-B column decreased slightly with increasing the flow rate, which maintained at 38 mg · mL-1 bed at a flow rate of 30 cm · min-1. In contrast, the dynamic capacity of IER-M column decreased drastically to a value of only 13 mg · mL-1 bed at a flow rate of 24 cm · min-1. Then, the IER-B column was used for the purification of molecular chaperonins GroEL and GroES. At elevated flow rates from 2.55 to 10.1 cm · min-1, the resolution and capacity of the column for GroEL and GroES purification was not affected by increasing flow rate. The results indicate that the biporous resin was promising for high-speed chromatographic purification of proteins.

Key Words

Column liquid chromatography Biporous ion exchange medium Protein Purification Molecular chaperonins 

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Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  1. 1.Department of Biochemical EngineeringSchool of Chemical Engineering and Technology Tianjin UniversityTianjinChina

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