, Volume 58, Issue 3, pp 231–234

Simultaneous Determination of Cefuroxime Axetil and Cefuroxime in Pharmaceutical Preparations by Thin-Layer Chromatography and Densitometry


DOI: 10.1365/s10337-003-0039-1

Cite this article as:
Krzek, J. & Dąbrowska-Tylka, M. Chromatographia (2003) 58: 231. doi:10.1365/s10337-003-0039-1


Conditions have been established for identification and quantification of cefuroxime axetil and cefuroxime by thin-layer chromatography and densitometry. Good separation of these compounds was achieved on silica gel by use of chloroform–ethyl acetate–glacial acetic acid–water, 4:4:4:1 (v/v), as mobile phase. UV densitometry was used to detect spots on chromatograms. Under these conditions the limits of detection for cefuroxime axetil and cefuroxime were 40 ng and 30 ng, respectively. Recoveries of cefuroxime axetil and cefuroxime were 99.93% and 97.94%, respectively.

Key Words

Thin-layer chromatography Densitometric detection Cefuroxime axetil and cefuroxime 

Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  1. 1.Department of Inorganic and Analytical ChemistryCollegium Medicum of Jagiellonian UniversityKrakówPoland

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