Abstract
In this paper, we demonstrate that in Physarum polycephalum, a naturally synchronized slime mold, histone deacetylase (HDAC) inhibitor Trichostatin A (TSA), arrestes the cell cycle at the checkpoints of S/G2, G2/M and mitosis exit, and influences the transcription of two ras genes Ppras1 and Pprap1, as well as the Ras protein level. Antibody neutralization experiment using anti-Ras antibody treatment showed that Ras protein played an important role in cell cycle checkpoint control through regulation of the level of Cyclin B1, suggesting that Ras protein might be a key factor for histone acetylation-mediated cell cycle regulation in P. polycephalum.
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Li, X., Lu, J., Zhao, Y. et al. Ras protein participated in histone acetylation-mediated cell cycle control in Physarum polycephalum . Chin.Sci.Bull. 50, 1721–1725 (2005). https://doi.org/10.1360/982005-307
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DOI: https://doi.org/10.1360/982005-307