Skip to main content
SpringerLink
Log in

Detection of genetically modified ingredients in seed samples from Heilongjiang soybean field

  • Articles
  • Published:
Chinese Science Bulletin

Abstract

A total of 210 soybean samples collected from different areas of Heilongjiang Province were analyzed by PCR to detect the presence of RR (Roundup Ready) soybean ingredient. Results showed that CaMV35S promoter was not detected in all samples. CP4-EPSPS gene was amplified in 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products. Further analysis by nested PCR indicated that there were no RR soybean ingredients in the samples. Besides, the amplified fragments of 0x1 by CP4-EPSPS primers were sequenced, and the results confirmed again the fact that this fragment was not from CP4-EPSPS gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Reference

  1. Quist, D., Chapela, I., Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico, Nature, 2001, 414: 541–543.

    Article  PubMed  CAS  Google Scholar 

  2. Mann, C. C., Has GM Corn invaded Mexico? Science, 2002, 295: 1617–1619.

    Article  PubMed  Google Scholar 

  3. Mann, C. C., Transgene data deemed unconvincing, Science, 2002, 296: 236–237.

    Article  PubMed  CAS  Google Scholar 

  4. http://www.foodqs.com/news/gjspzs01/20048215359.htm.

  5. Fan, Y. M., Research on correlative issues of imported soybean, Grain Distribution Technology (in Chinese), 2003, (6): 29–31.

  6. http://www.dadou.cn/read.aspx?id=372403.

  7. Wang, G. Y., The safety evaluation of transgenic plants, Journal of Agricultural Biotechnology (in Chinese), 2001, 9(3): 205–207.

    Google Scholar 

  8. Yang, C. J., Song, L., Wang, Z., Influence of transgenic soybeans on biodiversity, Environment Conservation (in Chinese), 2002, (11): 24–27.

  9. Wang, G. Y., Experiment Techniques of Genetic Engineering (in Chinese), Beijing: China Agricultural Science and Technology Press, 1997. 43–46.

    Google Scholar 

  10. Huang, K. L., Luo, Y. B., Detection genetically modified soybean Roundup Ready ingredient in foodstuffs by nested PCR and semi-nested PCR, Journal of Agriculture Biotechnology (in Chinese), 2003, (5): 461–466.

  11. Lü, S. H., Chang, R. Z., Tao, B., Methodological research on PCR based detection of genetically modified soybean resistant to glyphosate, Scientia Agriculture Sinica (in Chinese), 2003, (8): 883–887.

Download references

Author information

Authors and Affiliations

  1. Department of Seed Science and Technology, and State Key Lab of Agrobiotechnology, China Agricultural University, 100094, Beijing, China

    Jiang Haiyan, Wang Jianhua, Wu Peng, Li Yongchun & Wang Guoying

Authors
  1. Jiang Haiyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wang Jianhua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wu Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Li Yongchun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wang Guoying
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wang Jianhua.

About this article

Cite this article

Haiyan, J., Jianhua, W., Peng, W. et al. Detection of genetically modified ingredients in seed samples from Heilongjiang soybean field. Chin.Sci.Bull. 50, 975–979 (2005). https://doi.org/10.1360/982004-641

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1360/982004-641

Keywords

Search

Navigation