Abstract
We have searched the Bombyx mori genome for members of the major enzyme family, the Cytochrome P450s, which carry out multiple reactions to enable organisms to rid themselves of foreign compounds. As a result, 86 putative P450s were discovered in silkworm genome, which are thought to belong to 32 subfamilies. A comparative genomic analysis with Drosophila melanogaster reveals that the two insects have some similar P450 distribution patterns but still have some obvious differences. Especially, the diverse distribution exists in 7 p450 subfamilies, which are CYP4A, CYP4C, CYP4D, CYP6A, CYP6AE, CYP6B and CYP9A. Furthermore, we collected expression sequence tag (EST) evidence for 49 putative P450s genes, which are expressed at the transcriptional level and more likely to be true P450s.
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Hudgson, E., in Comprehensive Insect Physiology Biochemistry and Pharmacology (eds. Kerkut, G. A., Gilbert, L. C.), Oxford: Pergamon Press, 1985, Vol. 11, 647–712.
Stegeman, J. J., Livingstone, D. R., Forms and functions of cytochrome P450, Comp. Biochem. Physiol., 1998, 212C: 1–3.
Kulkarni, A. P., Hodgson, E., Metabolism of insecticides by mixed function oxidase systems, Pharmac. Ther., 1980, 8: 379–475.
Rendic, S., Di Carlo, F. J., Human cytochrome P450 enzymes: A status report summarizing their reactions, substrates, inducers, and inhibitors, Drug Metab. Rev., 1997, 29: 413–580.
Mansuy, D., The great diversity of reactions catalyzed by cytochromes P450, in Comparative Biochemistry and Physiology C Pharmacology Toxicology and Endocrinology, Oxford/Tarnytown/NY: Pergamon Press, 1998, Nov., 121(1–3): 5–14.
Rees, H. H., Biosynthesis of ecdysone, in Comprehensive Insect Physiology, Biochemistry and Pharmacology (eds. Kerkut, G. A., Gilbert, L. I.), vol. 7, New York: Pergamon Press, 1985, 185–248.
Schooley, D. A., Baker, F. C. Juvenile hormone biosynthesis, in Comprehensive Insect Physiology, Biochemistry and Pharmacology (eds. Kerkut, G. A., Gilbert, L. I.), vol. 7, New York: Pergamon Press, 1985, 363–390.
Scott, J. G., cytochrome P450 and insecticide resistance, Insect Biochem. Mol. Biol., 1999, 29: 757–777.
Schuler, M., The role of cytochrome P450 monooxygenases in plantinsect interactions, Plant Physiol., 1996, 112: 1411–1419.
Berge, J. B., Feyereisen, R., Amichot, M., Cytochrome P450 monooxygenases and insecticide resistance in insects, Philos. Trans. R. Soc. Lond. B. Biol. Sci., 1998, 353: 1701–1705.
Nelson, D. R., Koymans, L., Kamataki, T. et al., P450 superfamily: Update on new sequences, gene mapping, accession numbers and nomenclature, Pharmacogenetics, 1996, 6: 1–42.
Omura, T., Forty years of cytochrome P450, Biochem. Biophys. Res. Commun., 1999, 266: 690–698.
Nagaraju, J., Goldsmith, M. R., Silkworm genomics-progress and prospects, Current Science, 2002, 83:415–425.
Burge, C., Karlin, S., Prediction of complete gene structures in human genomic DNA, J. Mol. Biol., 1997, 268: 78–94.
Salamov, A. A., Solovyev, V. V., Ab initio gene finding in Drosophila genomic DNA, Genome Res., 2000, 10: 516–522.
Wang, J., Li, S., Zhang, Y. et al., Vertebrate gene predictions and the problem of large genes, Nature Rev. Genet., 2003, 4: 741–749.
Altschul, S. F., Gish, W., Miller, W. et al., Basic local alignment search tool, J. Mol. Biol., 1990, 215(3): 403–410.
Altschul, S. F., Madden, T. L., Schaffer, A. A. et al., Gapped BLAST and PSI-BLAST: A new generation of protein database search programs, Nucleic Acids Res., 1997, 25(17): 3389–3402.
Zhong, L., Wang, K., Tan, J. et al., Putative cytochrome P450 genes in rice genome (Oryza sativa L. ssp. Indica) and their EST evidence, Science in China, Ser. C, 2002, 45(5): 512–517.
Lagos-Quintana, M., Rauhut, R., Lendeckel, W. et al., Identification of novel genes coding for small expressed RNAs, Science, 2001, 294(5543): 853–858.
Adams, M. D., Celniker, S. E., Holt, R. A. et al., The genome sequence of Drosophila melanogaster, Science, 2000, 287: 2185–2195.
Tijet, N., Helvig, C., Feyereisen, R., The cytochrome P450 gene superfamily in Drosophila melanogaster: Annotation, intron-exon organization and phylogeny, Gene, 2001, 262: 189–198.
Consortium, C. E., Genome sequence of the nematode C. elegans: A platform for investigating biology, Science, 1998, 282: 2012–2018.
Daborn, P. J., Yen, J. L., Bogwitz, M. R. et al., A single P450 allele associated with insecticide Resistance in Drosophil, Science, 2002, 297:2253–2256.
Xia, Q., Zhou, Z., Lu, C. et al., A draft sequence for the genome of the domesticated silkworm (Bombyx mori), Science, 2004, 306(5703): 1937–1940.
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Bin, L., Xia, Q., Lu, C. et al. Analysis of cytochrome P450 genes in silkworm genome (Bombyx mori). Sci. China Ser. C.-Life Sci. 48, 414–418 (2005). https://doi.org/10.1360/04yc0057
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DOI: https://doi.org/10.1360/04yc0057