Skip to main content
SpringerLink
Log in

Study of binding glycyrrhetic acid to AT1 receptor

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

To analyze the binding of glycyrrhetic acid (GA) to angiotensin II type I (AT1) receptor and to explore the mechanisms underlying the binding, primary cell culture of rat vascular smooth muscle cell (VSMC), radioactive ligand-receptor binding assay, lascer confocal scanning microscope (LCSM), Northern blot, 3H-TdR incorporation DNA assay were used in this study. The results suggest that specific binding of GA to AT1 receptor (IC50 value was 35.0 μmol/L) increases intracellular [Ca2+]i of VSMC, activates transcription factor c-myc and promotes the proliferation of VSMC, therefore GA was probably an agonist of AT1 receptor, providing a new target for GA’s pharmaceutical effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hu, Z., Manufacturing and applying of Licorice acids medicine, Acta Pharmaceutica Sinica (in Chinese), 1988, 23(7): 553–560.

    CAS  Google Scholar 

  2. Metsarinne, K. P., Stoll, M., Falkenhahn, M. et al., Inhibiting the effects of angiotensin on cardiovascular hypertrophy, in Angiotensin Receptors (eds. Saavedra, J. M., Timmermans, P. B. M. W. M.), New York: Plenum Press, 1994, 235.

    Google Scholar 

  3. He, S., Jing, Y., Yue, B. et al., Binding of glycyrrhetinic acid to the angiotensin II receptor in rat liver membrane, Chinese Pharmacological Bulletin (in Chinese), 1998, 14(6): 519–521.

    CAS  Google Scholar 

  4. Liu, Y., Pleyte, K., Knaus, H. G. et al., Increased expression of Ca2+-sensitive K+ channels in aorta of hypertension rats, Hypertension, 1997, 30(6): 1403–1409.

    PubMed  CAS  Google Scholar 

  5. Rusch, N. J., Liu, Y., Pleyte, K. A., Mechanism for regulation of arterial tone by Ca2+-sensitive K+ channels hypertension, Clin-Exp-Pharmacol-Physiol, 1996, 23(12): 1077–1081.

    Article  PubMed  CAS  Google Scholar 

  6. Patel, M. K., Betteridge, L. J., Hughes, A. D. et al., Effect of angiotensin II on the expression of the early growth response gene c-fos and DNA Synthesis in human vascular smooth muscle cells, J. Hypertension, 1996, 14(3): 341–347.

    Article  CAS  Google Scholar 

  7. Brock, T. A., Alexander, R. W., Ekatein, L. S. et al., Angiotensin increases cytosolic free calcium in cultured vascular smooth muscle cell, Hypertension, 1985, 7: 1105–1109.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biophysics, Health Science Center of Peking University, 100083, Beijing, China

    Fengyun Zhang & Shipeng He

  2. School of Pharmaceutical Science, Health Science Center of Peking University, 100083, Beijing, China

    Baozhen Yue

Authors
  1. Fengyun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Baozhen Yue
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shipeng He
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fengyun Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, F., Yue, B. & He, S. Study of binding glycyrrhetic acid to AT1 receptor. Sci. China Ser. C.-Life Sci. 46, 194–204 (2003). https://doi.org/10.1360/03yc9021

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1360/03yc9021

Keywords

Search

Navigation