Abstract
The electrooxidation of 3,3′, 5,5′-tetramethylbenzidine (TMB) is dependent on the pH value of Britton-Robinson (B-R) buffer solution. In this work, the electrooxidation behavior of TMB was investigated with a SnO2:F film optically transparent thin-layer spectroelectrochemical cell. TMB underwent one two-electron electrooxidation process in the pH range from 2.0 to < 4.0, and two successive one-electron electrooxidation processes in the pH range from 4.0 to < 7.0 in the B-R buffer solution. At pH 6.5, the electrooxidative product of TMB generated a subsequent chemical reaction to yield an azo compound. Several spectroelectrochemical techniques, such as thin-layer cyclic voltammetry, thin-layer cyclic voltabsorptometry, thin-layer potential-controlled electrolysis absorptometry, thin-layer single-potential-step chronoabsorptometry, thin-layer double-potential-step chronoabsorptometry, thin-layer single-potential-step open-circuit relaxation chronoabsorptometry, were applied to this investigation. The formal potential E0′ and the electron transfer number corresponding to the electrooxidation of TMB in B-R buffer solution, and the reaction rate constant of the subsequent chemical reaction were determined.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wang Tong, Sun Wei, Jiao Kui, Studies on the voltammetric enzyme-linked immunoassay system based on 3,3′,5,5′-tetrame-thylbenzidine (TMB) as substrate, Chinese Journal of Analytical Chemistry (in Chinese), 2002, 30(11): 1298–1302.
He Yanan, Chen Hongyuan, 3,3′,5,5′-Tetramethylbenzidine (TMB)-H2O2-HRP voltammetric immunoassay for the determination of IgE in human serum, Chem. J. Chinese Universities (in Chinese), 1997, 18(8): 1306–1308.
Jiao Kui, Zhang Shusheng, Sun Wei, Authorization Certificate of Science and Technology of Shandong Province, 1999, No: 197.
Jiao Kui, Lu Gang, Yang Tao et al., Electrochemical and spectroelectrochemical study of m-phenylenediamine, Chem. J. Chinese Universities (in Chinese), 2003, 24(6): 1005–1008.
Mohr, P., Harke, T., Kuhn, W. et al., Mechanism studies of enzymatically formed tolidine blue and determination of peroxidatic activities, Biomed. Biochim. Acta, 1983, 42(6): 663–672.
Matrka, M., Pipalova, J., Semiquinone formation during the oxidation of benzidine, o-tolidine, and o-dianisidine, Chem. Prum., 1971, 21(1): 14–18.
Shen Baoen, Wang Guiliang, Thin-layer spectroelectrochemical investigation on indigo carmine, Acta Physico-Chimica Sinica (in Chinese), 1990, 6(1): 71–76.
Jiao Kui, Zhang Shusheng, Wei Lu et al., Investigation of voltammetric enzyme-linked immunoassay system based on new system of OT- H2O2-HRP, Acta Chimica Sinica (in Chinese), 1997, 55: 1121–1129.
Wang Huawei, Shen Baoen, Peng Tuzhi et al., The mechanism of electrooxidation of o-tolidine, J. of Hangzhou Universities (in Chinese), 1994, 21(2): 198–203.
Cheng, G, Yang, Y, Dong, S., Spectroelectrochemical study of biliverdin produced by the electrooxidation of bilirubin. Bioelectrochem. Bioenerg., 1991, 26: 35–39.
Dong Shaojun, Song Shihua, Cheng Guangjin, Optically thin-layer spectroelectrochemical investigation on murexide electroreduction progress, Acta Physico-Chimica Sinica (in Chinese), 1987, 3(4): 368–373.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jiao, K., Yang, T. & Niu, S. A thin-layer spectroelectrochemical study of 3,3′,5,5′-tetramethylbenzidine at SnO2:F film optically transparent electrode. Sc. China Ser. B-Chem. 47, 267–275 (2004). https://doi.org/10.1360/03yb0174
Received:
Issue Date:
DOI: https://doi.org/10.1360/03yb0174