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Preparation of tryptophan modified chitosan beads and their adsorption of low density lipoprotein

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Chinese Science Bulletin

Abstract

An adsorbent with good adsorption performance for binding low density lipoprotein (LDL) was synthesized through a simple preparation procedure. The phase-inversion technique was employed to form macroporous chitosan beads, which were then simultaneously activated, cross-linked and connected with a long spacer arm via the reaction with ethyleneglycol diglycidylether (EGDE). Tryptophan (Trp) was subsequently coupled to the epoxy-activated beads. This adsorbent demonstrated satisfactory adsorption capacity and selectivity for LDL, and it also exhibited excellent blood compatibility. The effect of spacer arms with different length on the adsorption selectivity was investigated experimentally, and the mechanism was analyzed.

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Authors and Affiliations

  1. Institute of Polymer Chemistry, The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Nankai University, 300071, Tianjin, China

    Guoqi Fu, Keyu Shi, Zhi Yuan & Binglin He

  2. Tianjin Medical College, 300052, Tianjin, China

    Bin Liu, Bin Shen & Qishun Wang

Authors
  1. Guoqi Fu
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  2. Keyu Shi
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  3. Zhi Yuan
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  4. Binglin He
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  5. Bin Liu
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  6. Bin Shen
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  7. Qishun Wang
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Correspondence to Guoqi Fu.

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Fu, G., Shi, K., Yuan, Z. et al. Preparation of tryptophan modified chitosan beads and their adsorption of low density lipoprotein. Chin.Sci.Bull. 48, 2303–2307 (2003). https://doi.org/10.1360/03wb0052

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  • DOI: https://doi.org/10.1360/03wb0052

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