Abstract
Ten different isolates of a carlavirus were detected by degenerate PCR from 12 garlic samples collected from 6 provinces in China, and the complete genome sequence of the Zhejiang isolate ZJ1 and 3’-terminal sequences of 9 other isolates were determined. The RNA genome of isolate ZJ1 consisted of 8363nts excluding the 3’-poly (A) tail, and the genome organization was similar to other carlaviruses with 6 open reading frames encoding a replicase, TGB1, TGB2, TGB3, CP and NABP respectively. Sequence comparisons showed that all 10 isolates were Garlic latent virus (GarLV). The variations in the TGB2, TGB3 and NABP were more significant than those in the CP. High homology was also detected between those isolates and Shallot latent virus (ShLV). Phylogenetic analysis suggested that GarLV isolates from garlic can be divided into 4 main groups and Chinese isolates belonged to each group. This is the first reported molecular analysis of members of the genus Carlavirus in China.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Van Dijk, P., Carlavirus isolates from cultivated Allium species represent three viruses, Neth. J. Plant Pathology, 1993, 99: 233–257.
Tsuneyoshi, T., Sumi, S., Differentiation among garlic viruses in mixed infections based on RTfPCR procedures and direct tissue blotting immunoassays, Phytopathol., 1996, 86: 253–259.
Tsuneyoshi, T., Matsumi, T., Deng, T. C. et al., Differentiation of allium carlaviruses isolated from different parts of the world based on the viral coat protein sequence, Arch. Virol., 1998, 143: 1093–1107.
Xu, P. W., Shun, H. S., Shun, R. J. et al., Development and application of garlic virus eradication technology, Chinese Agricultural Sciences (in Chinese), 1998, 31(2): 92–93.
Foster, G. D., Carlavirus isolation and RNA extraction, Methods Mol. Biol., 1998, 81: 145–150.
Yang, J. P., Chen, J. P., Chen, J. et al., Sequence analysis of a soil-borne wheat mosaic virus isolate from Italy shows that it is the same virus as European wheat mosaic virus and soil-borne rye mosaic virus, Science in China, Ser. C, 2001, 44(2): 216–224.
Genetics Computer Group, Wisconsin Package version 10.0. 575 Science Drive, Madison, Wisconsin, USA 53711, 1998.
Chen, J., Chen, J. P., Adams, M. J., A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family, Arch. Virol., 2001, 146(4): 757–766.
Van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L. et al., Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, London, San Diego: Academic Press, 2000, 969–975.
Wong, S. M., Lee, K. C., Yu, H. H. et al., Phylogenetic analysis of triple gene block viruses based on the TGB 1 homology gene indicates a convergent evolution, Virus Genes, 1998, 16: 295–302.
Morozov, S. Y., Solovyev, A. G., Kalinina, N. O. et al., Evidence for two nonoverlapping functional domains in the potato virus X 25K movement protein, Virology, 1999, 260(1): 55–63.
Wung, C. H., Hsu, Y. H., Liou, D. Y. et al., Identification of the RNA-binding sites of the triple gene block protein 1 of bamboo mosaic potexvirus, J. Gen. Virol., 1999, 80: 1119–1126.
Voinnet, O., Lederer, C., Baulcombe, D. C., A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana, Cell, 2000, 103: 157–167.
Cruz, S. S., Roberts, A. G., Prior, D. A. et al., Cell-to-cell and phloem-mediated transport of potato virus X: The role of virions, Plant Cell, 1998, 10: 495–510.
Solovyev, A. G., Stroganova, T. A., Zamyatnin, A. A. et al., Subcellular sorting of small membrane-associated triple gene block proteins: TGBp3-assisted targeting of TGBp2, Virology, 2000, 269(1): 113–127.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chen, J., Zheng, H., Chen, J. et al. Genome organization and variation in the 3′-partial sequence of garlic latent virus in China. Sci. China Ser. C.-Life Sci. 45, 441–448 (2002). https://doi.org/10.1360/02yc9049
Received:
Issue Date:
DOI: https://doi.org/10.1360/02yc9049