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PKCα translocation from mitochondria to nucleus is closely related to induction of apoptosis in gastric cancer cells

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Abstract

PKCs have been implicated in the regulation of cellular differentiation, proliferation, apoptosis and signal transduction. It was demonstrated in this study that PKCα was located both at mitochondria and in cytosol in gastric cancer cell line BGC-823. Treatment of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in the translocation of PKCα from both mitochondria and cytosol to nucleus as clearly shown by laserscanningconfocal microscopy, while the protein level of PKCα was not changed by TPA treatment as detected by Western blot. The results also revealed that TPA-induced translocation of PKCα was in close association with apoptosis induction, and such association was further affirmed by other experiments where various apoptotic stimuli and specific inhibitors of PKC were used. Taken together, these findings indicate that translocation of PKCα from both mitochondria and cytosol to nucleus in gastric cancer cell is accompanied by induction of apoptosis, and may imply a new mechanism of the potential linking between cell apoptosis and PKCα translocation.

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, 361005, Xiamen, China

    Wu Qiao, Liu Su, Ding Liang, Ye Xiaofeng & Su Wenjin

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  1. Wu Qiao
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  2. Liu Su
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  3. Ding Liang
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  4. Ye Xiaofeng
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  5. Su Wenjin
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Correspondence to Wu Qiao.

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Qiao, W., Su, L., Liang, D. et al. PKCα translocation from mitochondria to nucleus is closely related to induction of apoptosis in gastric cancer cells. Sci. China Ser. C.-Life Sci. 45, 237–244 (2002). https://doi.org/10.1360/02yc9026

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  • DOI: https://doi.org/10.1360/02yc9026

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