Abstract
Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young’s Modulus but not change their apparent height; (iii) no detectable change in either height or Young’s Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.
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Qingqing, W., Xiaoyang, C., Keying, C. et al. Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states. Sci. China Ser. C.-Life Sci. 45, 225–236 (2002). https://doi.org/10.1360/02yc9025
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DOI: https://doi.org/10.1360/02yc9025