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Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy

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Abstract

By using environmental scanning electron microscopy, the morphological changes of Vero cells attached to and grown on the microcarrier Cytodex-3 were observed, and their behavior of adhesion, spreading and proliferation was analyzed. The effect of exogenous fibronectin/ laminin on adhesion and spreading of MCC/Vero cell was studied. The images of ESEM showed that expansion of cell growth was directed toward vacancy space. The growth curve and cell concentration change during the whole culture process were obtained from the statistical counting method based on ESEM images and the crystal violet method. The growth rate of Vero cells increases with increasing the concentration of cell inoculation, that is, the specific growth rate increases quickly with increasing the concentration of cell inoculation. When serum concentration in medium #199 ranged from 5% to 10%, experimental results indicated that serum concentration is one of the important factors influencing cell growth, particularly in the cell adhesion and spreading stage.

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Authors and Affiliations

  1. Multiphase Reaction Laboratory, Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080, Beijing, China

    Manjun Shao & Lei Jiang

  2. State Key Laboratory of Biochemical Engineering, Chinese Academy of Sciences, 100080, Beijing, China

    Wei Cong & Fan Ouyang

Authors
  1. Manjun Shao
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  2. Lei Jiang
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  3. Wei Cong
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  4. Fan Ouyang
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Correspondence to Manjun Shao.

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Shao, M., Jiang, L., Cong, W. et al. Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy. Sci. China Ser. C.-Life Sci. 45, 149–158 (2002). https://doi.org/10.1360/02yc9017

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  • DOI: https://doi.org/10.1360/02yc9017

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