Skip to main content
SpringerLink
Log in

Establishment of multiple shoot clumps from maize (Zea mays L.) and regeneration of herbicide-resistant transgenic plantlets

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

A kind of quick, efficient and season-free inducing embryoid and multiple shoot clumps system from shoot tip meristems that derived from elite inbreds of maize was established. The herbicide-resistant gene als (coding Acetolactate synthase) isolated from a mutant of Arabidopsis thaliana was transferred to tissue pieces of maize multiple shoot clumps by microprojectile bombardment. Herbicide-resistant tissue and regenerants were obtained through selections with herbicide chlorsulfuron. PCR analysis and Southern blot hybridization indicated that gene als has been transferred to some regenerants. The test of spraying chlorsulfuron displayed that the transgenic plantlets and R1 plants had favorable herbicide-resistant trait. We have established a new genotype-free system of maize which could rapidly and efficiently produce large quantities of transgenic plantlets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fromm, M. E., Morrish, F., Armstrong, C. et al., Inheritance and expression of chimeric genes in the progeny of transgenic maize plants, Bio/Technology, 1990, 8: 833–839.

    Article  PubMed  CAS  Google Scholar 

  2. Gordon-Kamm, W. J., Spencer, M. T., Mangano, M. L. et al., Transformation of maize cells and regeneration of fertile transgenic plants, Plant Cell, 1990, 2: 603–618.

    Article  PubMed  CAS  Google Scholar 

  3. Walters, D. A., Vetsch, C. S., Potts, D. E. et al., Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants, Plant Mol. Biol., 1992, 18: 189–200.

    Article  PubMed  CAS  Google Scholar 

  4. Koziel, M. G., Beland, G. L., Bowman, C. et al., Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis, Bio/Technology, 1993, 11: 194–200.

    Article  CAS  Google Scholar 

  5. Brettschneider, R., Becker, D., Lörz, H., Efficient transformation of scutellar tissue of immature maize embryos, Theor. Appl. Genet., 1997, 94: 737–748.

    Article  CAS  Google Scholar 

  6. Wan, Y., Widholm, J. M., Lemaux, P. G., Type I callus as a bombardment target for generating fertile transgenic maize (Zea mays L.), Planta, 1995, 196: 7–14.

    Article  CAS  Google Scholar 

  7. Wang, G. Y., Du, T. B., Zhang, H. et al., Transfer of Bt-toxin protein gene into maize by high-velocity microprojectile bombardments and regeneration of transgenic plants, Science in China, Ser. B, 1995, 38(8): 817–824.

    Google Scholar 

  8. Wang, S. C., Wang, G. Y., Ding, Q. X. et al., Studies of transgene segregation and integration in maize, Acta Genetica Sinica (in Chinese), 1999, 26(3): 254–261.

    CAS  Google Scholar 

  9. Li, S. R., Zhang, J. R., Chen, H. M., Study on induction of embryogenic callus and plantlets regeneration in maize, Journal of Shandong University (in Chinese), 1990, 25(1): 116–124.

    Google Scholar 

  10. Xiang, F. N., Zhang, J. R., Chen, H. M. et al., Long-term culture and chromosome variations of embryogenic calli in maize, Acta Bot. Boreal-Occident Sin. (in Chinese), 1994, 14(3): 157–163.

    Google Scholar 

  11. Gordon-Kamm, W. J., Baszczynski, C. L., Bruce, W. B. et al., Transgenic cereal-Zea mays (maize), in Molecular Improvement of Cereal Crops (ed. Vasil, I. K.), Dordrecht: Kluwer Academic Publishers, 1999, 189–253.

    Google Scholar 

  12. Santos, J. M., Torne, J. L., Methods of obtaining maize totipotent tissues (I)—Seedling segments culture, Plant Sci. Lett., 1984, 33: 309–315.

    Article  Google Scholar 

  13. Zhong, H., Srinivasan, C., Mariam, B. S., In-vitro morphogenesis of corn (Zea mays L.) (I)—Differentiation of multiple shoot clumps and somatic embryos from shoot tip, Planta, 1992, 187: 483–489.

    Article  CAS  Google Scholar 

  14. Lowe, K., Bowen, B., Hoerster, G. et al., Germline transformation of maize following manipulation of chimeric shoot meristems, Bio/Technology, 1995, 13: 677–682.

    Article  CAS  Google Scholar 

  15. Li, X. H., Zhang, J. R., Direct differentiation of ears and tassels from cultured shoot apices of maize, Science in China, Ser. C, 1999, 42(1): 17–24.

    PubMed  CAS  Google Scholar 

  16. Li, G. S., Yang, A. F., Zhang, J. R. et al., Genetic transformation of calli from maize and regeneration of herbicide-resistant plantlets, Chinese Science Bulletin, 2001, 46(7): 563–565.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Life Science, Shandong University, 250100, Jinan, China

    Li Guosheng, Zhang Qingwei & Zhang Juren

  2. Biotechnique Research Center, Shandong Agriculture Science Institute, 250100, Jinan, China

    Bi Yuping & Shan Lei

Authors
  1. Li Guosheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhang Qingwei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhang Juren
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bi Yuping
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shan Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhang Juren.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, G., Zhang, Q., Zhang, J. et al. Establishment of multiple shoot clumps from maize (Zea mays L.) and regeneration of herbicide-resistant transgenic plantlets. Sci. China Ser. C.-Life Sci. 45, 40–49 (2002). https://doi.org/10.1360/02yc9005

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1360/02yc9005

Keywords

Search

Navigation