Abstract
To reveal the mutation effect of low-energy ion implantation on Ambidopsis thaliana in vivo, T80II, a stable dwarf mutant, derived from the seeds irradiated by 30 keV N+ with the dose of 80 X 1015 ions/cm2 was used for Random Amplified Polymorphic DNA (RAPD) and base sequence analysis. The results indicated that among total 397 RAPD bands observed, 52 bands in T80II were different from those of wild type showing a variation frequency 13.1%. In comparison with the sequences of A. thaliana in GenBank, the RAPD fragments in T80II were changed greatly in base sequences with an average rate of one base change per 16.8 bases. The types of base changes included base transition, transversion, deletion and insertion. Among the 275 base changes detected, single base substitutions (97.09%) occurred more frequently than base deletions and insertions (2.91%). And the frequency of base transitions (66.55%) was higher than that of base transversions (30.55%). Adenine, thymine, guanine or cytosine could be replaced by any of other three bases in cloned DNA fragments in T80II. It seems that thymine was more sensitive to the irradiation than other bases. The flanking sequences of the base changes in RAPD fragments in T80II were analyzed and the mutational “hotspot” induced by low-energy ion implantation was discussed.
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References
The Arabidopsis Genome Initiative, Analysis of the genome sequence of the flowering plant Arabidopsis thaliana, Nature, 2000, 408(6814): 796–815.
Tanaka, A., Shikazono, N., Yokota, Y. et al., Effects of heavy ions on the germination and survival of Arabidopsis thaliana, International Journal of Radiation Biology, 1997, 72(1): 121–127.
Yu, Z. L., Ion beam application in genetic modification, IEEE Transactions on Plasma Science, 2000, 28(1): 128–132.
Wu, L. F., Yu, Z. L., Radiobiological effects of a low-energy ion beam on wheat, Radiation and Environmental Biophysics, 2001, 40(1): 53–57.
Du, Y. H., Huang, S. H., Tan, Z. et al., Determination of DNA single-strand breaks by low-energy heavy ion and analysis of dose-effect curves, Chinese Science Bulletin, 1999, 44(8): 711–714.
Li, L., Yang, J. B., Li, J. et al., Research on damage of ion beam irradiated pUC18 plasmid DNA, Journal of Anhui Agricultural Sciences (in Chinese with the English Abstract), 1994, 22(4): 300–303.
Yang, J. B., Wu, L. J., Li, L. et al., Sequence analysis of lacZ − mutations induced by ion beam irradiation in double-stranded M13mp18 DNA, Science in China, Ser. C, 1997, 40(1): 107–112.
Williams, J. D. K., Kubelik, A. R., Livak, K. J. et al., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Research, 1990, 18(18): 6531–6535.
Yu, Z. L., Deng, J. G., He, J. J. et al., Mutation breeding by ion implantation, Nuclear Instruments and Methods in Physics Research, 1991, B59/60: 705–708.
Rogers, S. O., Bendich, A. J., Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues, Plant Molecular Biology, 1985, 5(2): 69–76.
Zhang, Z. H., Du, L. Q., Li, Y. X. et al., The variations of M1 to the seeds of tobacco implanted with ion beam, Acta Biophysica Sinica (in Chinese with the English Abstract), 1998, 14(4): 762–766.
Shao, C. L., Yu, Z. L., Research into releasing inorganic phosphate and base from 5′-dTMP irradiation by a low energy ion beam, Radiation Physics and Chemistry, 1994, 44(6): 651–654.
Miller, J. H., Mutagenic specificity of ultraviolet light, Journal of Molecular Biology, 1985, 182(1): 45–68.
Tindall, K. R., Stein, J., Hutchinson, F., Changes in DNA base sequence induced by Gamma-ray mutagenesis of lambda phage and prophage, Genetics, 1988, 118(4): 551–560.
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Chang, F., Liu, X., Li, Y. et al. Changes in DNA base sequences in the mutant of Arabidopsis thaliana induced by low-energy N+ implantation. Sci. China Ser. C.-Life Sci. 46, 503–512 (2003). https://doi.org/10.1360/02yc0177
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DOI: https://doi.org/10.1360/02yc0177