Skip to main content
SpringerLink
Log in

Syntheses of polyamidoamine dendrimers starting from a hexadimensional core and application in gene transfer

  • Published:
Science in China Series B: Chemistry Aims and scope Submit manuscript

Abstract

Polyamidoamine dendrimers using inositol as a core were designed, synthesized and characterized. The electrostatics interaction of dendrimer with DNA causes the condensation of DNA to form DNA/dendrimer complexes. The sizes of complexes prepared from dendrimer G6 and pRE Luc at N/P ratio 10: 1 range from 80 to 300 nm. Dendrimers G3—G6 are able to efficiently deliver pRE Luc and pSV β-gal gene into HeLa and HEK 293 cells. The transfection efficiencies of G5 and G6 are much higher than that of poly L-lysine (PLL) and comparable to that of the commercial branched polyethylenimine (PEI, Mw 25000), whereas the cytoctoxicities of G5 and G6 are lower than that of PLL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Culver, W., Gene Therapy: A Primer for Physician, Larchmont: Mary Ann Liebert, Inc., 1996, xvi.

    Google Scholar 

  2. Mahato, R. I., Smith, L. C., Rolland, A., Pharmaceutical perspective of nonviral gene therapy, Adv. Genet., 1999, 41: 95–156.

    Article  CAS  Google Scholar 

  3. Anderson, W. F., Human gene therapy, Nature, 1998, 392(Supp.): 25–30.

    CAS  Google Scholar 

  4. Luo, D., Saltzman, M., Synthetic DNA delivery systems, Nat. Biotech., 2000, 18: 33–37.

    Article  CAS  Google Scholar 

  5. Han, S., Mahato, R. I., Sung, Y. K. et al., Development of biomaterials for gene therapy, Mol. Ther., 2000, 2(4): 302–317.

    Article  CAS  Google Scholar 

  6. Malik, N., Wiwattanapatapee, R., Klopsch, R. et al., Dendrimers: relationship between structure and biocompatibility in vitro, and preliminary studies on the biodistribution of 125I-labelled polyamidoamine dendrimers in vivo, J. Control. Release, 2000, 65(1–2): 133–148.

    Article  CAS  Google Scholar 

  7. Esfand, R., Tomalia, D. A., Poly(amidoamine)(PAMAM) dendrimers: from biomimicry to drug delivery and biomedical applications, Drug. Discov. Today, 2001, 6(8): 427–436.

    Article  CAS  Google Scholar 

  8. Zhuo, R. X., Du, B., Lu, Z. R., In vitro release of 5-fluorouracil with cyclic core dendritic polymer, J. Controll. Release, 1999, 57: 249–257.

    Article  CAS  Google Scholar 

  9. Haensler, J., Szoka, F. Jr., Polyamidoamine cascade polymers mediate efficient transfection of cells in culture, Bioconjug. Chem., 1993, 4(5): 372–379.

    Article  CAS  Google Scholar 

  10. Kukowska-Latallo, J. F., Bielinska, A. U., Johnson, J. et al., Efficient transfer of genetic material into mammalian cells using starburst polyamidoamine dendrimers, Proc. Natl Acad. Sci. USA, 1996, 93(10): 4897–902.

    Article  CAS  Google Scholar 

  11. Du, Zhou, R. J., Zhuo, R. X., Synthesis of cyclic core dendritic polymer and its usage as a vector for transfer foreign DNA into Human cells, Chin. Chem. Lett., 1999, 9: 635–638.

    Google Scholar 

  12. Cheng, H., Zhou, R., Liu, L. et al., Cyclic core dendrimer as a new kind of vector for gene transfer into mammalian cells, Genetica, 2000, 108(1): 53–56.

    Article  CAS  Google Scholar 

  13. Guo, L., Weng, L., Zheng, H., Synthesis of hexakis(ω-hydroxypropyl) ether of inositol and derivatives, Chin. J. Org. Chem., 1995, 15(3): 308–311.

    CAS  Google Scholar 

  14. Hansen, M., Nielsen, S. E., Berg, Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill, J. Immunol. Methods, 1989, 119: 203–210.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Wuhan University, 430072, Wuhan, China

    Shiwen Huang & Xueqing Zhang

  2. College of Life Sciences, Wuhan University, 430072, Wuhan, China

    Liezhen Fu, Hui Liu, Wenxin Li & Renxi Zhuo

Authors
  1. Shiwen Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liezhen Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xueqing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wenxin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Renxi Zhuo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Renxi Zhuo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, S., Fu, L., Zhang, X. et al. Syntheses of polyamidoamine dendrimers starting from a hexadimensional core and application in gene transfer. Sc. China Ser. B-Chem. 46, 271–279 (2003). https://doi.org/10.1360/02yb0090

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1360/02yb0090

Keywords

Search

Navigation