Abstract
The chromosomes 1, 3, 5, 6, 7, 10 and 12 of rice field eel (Monopterus albus Zuiew) have been microdissected successfully from meiosis I diakinesis spreads by using glass microneedle under an inverted microscope. And the DOP-PCR products of the single chromosome dotted on the nylon membrane as “specific chromosomal DNA pool”, have been hybridized with 6 probes to map these genes. The mapping results show that Zfa has been mapped to chromosome 1, rDNA to chromosomes 3 and 7, both Gh and Pdegy to chromosome 10, Hsl to chromosome 5 and Hox genes have been detected on chromosomes 1, 3, 6 and 10 meantime. It has initiatively been suggested that chromosome 10 of rice field eel might possess the commom conserved synteny to that on chromosome 17 of human, chromosome 11 of mouse, chromosome 12 of pig and chromosome 19 of bovine. And so chromosome 3 of rice field eel might also contain the commom conserved synteny to that on chromosome 2 of zebrafish. Our study is an attempt to establish a new and feasible method to advance the study of gene mapping and chromosome evolution in fish, and also to provide a new idea to distinguish each chromosome on the base of molecular markers for fish.
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Toder, R., Wienberg, J., Voullaire, L. et al., Shared DNA sequences between the X and Y chromosomes in the tammar wallaby-evidence for independent additions to eutherian and marsupial sex chromosomes, Chromosoma, 1997, 106: 94.
Weimer, J., Kiechle, M., Senger, G. et al., An easy and reliable procedure of microdissection technique for the analysis of chromosomal breakpoints and marker chromosomes, Chromosome Research, 1999, 7: 355.
Boni, R., Xin, H., Hohl, D. et al., Syringocystadenoma papilliferum: A study of potential tumor suppressor genes, Am J. Dermatopathol., 2001, 23(2): 87.
Akiyama, M., Kawame, H., Ohashi, H. et al., Functional disomy for Xq26.3-qter in a boy with an unbalanced t (X; 21) (q26.3; p11.2) translocation, Am. J. Med. Genet., 2001, 99(2): 111.
Xia, J. H., Yang, Y., Dai, H. P. et al., Construction of 14 DNA probe for specific chromosomal bands, Acta Genetica Sinic, 1994, 21(4): 253.
Song, W. Q., Cui, X. Q., Xu, W. S. et al., Microdissection and PCR of the terminal part of big M chromosome long arm from horsebean, Chinese Science Bulletin (in Chinese), 1996, 41(4): 361.
Dang, B. Y., Hu, Z. M., Zhou, Y. H. et al., Construction of single chromosome DNA library from lilium regale Wilson, Chinese Science Bulletin, 1998, 43(5): 434.
Cheng, Z. K., Yan, H. H., Dang, B. Y. et al., Microdissection and amplification of the chromosome arm 5s in a rice telotetrasomic, Chinese Science Bulletin, 1998, 43(7): 590.
Tian, C., Lu, Y. F., Deng, J. F. et al., The cloning of HMW-GS1Dx5 gene fragment using chromosome microdissection in wheat, Chinese J. of Biotechnology, 1999, 15(2): 255.
Liu, B., Rong, J. K., Dong, Y. S. et al., Microdissection of chromosome 7B of common wheat and cloning of low-copy specific DNA sequences, Chinese Science Bulletin, 1999, 44(7): 632.
Yu, Q. X., Fan, L. C., Cui, J. X. et al., High resolution G-banding and idiogram on pachytene bivalents of rice field eels (Monoptrus albus Zuiew), Science in China, ser. B, 1994, 37(9): 1091.
Telenius, H., Carter, N. P., Bebb, C. E. et al., Degerate oligonucleotide-primed PCR: general amplification or target DNA by a single degerate primer, Genomics, 1992, 144: 737.
Christian, A. T., Garcia, H. E., Tucker, J., PCR in situ followed by microdissection allows whole chromosome painting probes to be made from single microdissected chromosomes, Mammalian Genome, 1999, 10: 628.
Li, K., Yu, Q. X., Mao, Y. et al., Localization of rDNA genes of rice field eels (Monoptrrus albus Zuiew) on the bivalents 3q12-q24 and 7q14-26 by digoxigenin labeled in situ hybridization, Acta Genetica Sinica, 1995, 22(2): 97.
Yang, Y. P., Womack, J. E., Human chromosome 17 comparative anchor loci are conserved on bovine chromosome 19, Genomics, 1995, 27: 293.
Amores, A., Force, A., Yan, Y. L. et al., Zebrafish Hox clusters and vertebrate genome evolution, Science, 1998, 282: 171.
Macrae, A. D., Brenner, S., Analysis of the dopamine receptor family in the compact genome of the puffer fish Fugu rubripes, Genomics, 1995, 25(2): 436.
Mason, P. J., Stevens, D. J., Luzzatto, L. et al., Genomic structure and sequence of the Fugu rubripes glucose-6-phosphate dehydrogenase gene (G6PD), Genomics, 1995, 26(3): 587.
Elgar, G., Rattray, F., Greystrong, J. et al., Genomic structure and nucleotide sequence of the p55 gene of the puffer fish Fugu rubripes, Genomics, 1995, 27(3): 442.
Baxendale, S., Abdulla, S., Elgar, G. et al., Comparative sequence analysis of the human and pufferfish Huntington’s disease genes, Nat. Genet., 1995, 10(1): 67.
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Ji, F., Yu, Q. & Liu, J. Development of specific chromosomal DNA pool for rice field eel and their application to gene mapping. Chin.Sci.Bull. 47, 485–489 (2002). https://doi.org/10.1360/02tb9112
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DOI: https://doi.org/10.1360/02tb9112