Subcellular distribution and compartmentalization of arsenic in Pteris vittata L.
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The subcellular distribution of arsenic (As) in Pteris vittata L., an As-hyperaccumulator, was studied to determine As compartmentalization and to explore the mechanisms that confer As tolerance. When the plant was grown in a nutrient solution without additional As, most of the accumulated As was isolated to the cell wall. However, in plants growing in a nutrient solution containing 0.1 or 0.2 mmol/L As, approximately 78% of the total As accumulated within the pinna. The proportions of As accumulation in the cytoplasmic supernatant fraction were 78% of that in the pinna and 61% of that in the plant. In either treatment group (0.1 or 0.2 mmol/L As), the fraction containing the lowest level of As was the organelle fraction. These results suggest that As accumulates in the pinna where it is primarily distributed in the cytoplasmic supernatant fraction. The role of As compartmentalization may be intricately linked with As detoxification in P. vittata L.
Keywordsarsenic (As) compartmentalization detoxification hyperaccumulator Pteris vittata L. subcellular distribution
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- 1.Berry, W. L., Plant and factors influencing the use of plant analysis as a tool for biogeochemical prospecting, in Mineral Exploration: Biogeological Systems and Organic Matter (eds. Carlise D, Berry W L, Kaplan I R, et al.), New Jersey: Prentice-Hall, Englewood Cliffs, 1986, 5: 13.Google Scholar
- 2.Feed Additive Compendium (FAC), Munneapolis, America: Miller Publishing Company, 1975, 13: 330.Google Scholar
- 6.Chen, T. B., Huang, Z. C., Huang, Y. Y. et al., Distributions of arse- nic and essential elements in pinna of arsenic hyperaccumulator Pteris vittata L., Science in China, Ser. C, 2005, 48(1): 13–19.Google Scholar
- 7.Huang, Z. C., Screening of arsenic hyperaccumulator and the study on its accumulation mechanisms, Dissertation for the Doctoral De- gree, Beijing: Institute of Geographical Sciences and Natural Re- sources Research, Chinese Academy of Science, 2003, 109 - 129.Google Scholar
- 8.Li, W. X., Chen, T. B., Chen, Y. et al., Role of trichome of Pteris vittata L. in arsenic hyperaccumulation, Science in China, Ser. C, 2005, 48(2): 148–154.Google Scholar
- 14.Liao, B., Deng, D. M., Yang B. et al., Subcellular distribution and chemical forms of Cu in Commelina communis, Acta Scientiarum Naturalium Universitatis Sunyatseni (in Chinese), 2004, 43(2): 72–80.Google Scholar
- 15.Wan, M., Zhou, W., Lin, B., Subcelluar and molecular distribution of cadmium in two wheat genotypes differing in shoot/root Cd par- titioning, Scientia Agricultura Sinica (in Chinese), 2003, 36(6): 671–675.Google Scholar
- 16.Zhou, W., Wang, H., Lin, B., Effects of calcium supply on subcel- lular distribution of cadmium, chloroplast ultrastructure, RuBPC and PEPC activity in maize under cadmium stress, Plant Nutrition and Fertilizer Science, 1999, 5(4): 335–340.Google Scholar
- 17.Ni, T. H., Wei, Y. Z., Subcellular distribution of cadmium in mining ecotype Sedum alfredii, Acta Botanica Sinica, 2003, 45(8):925- 928.Google Scholar
- 19.Hans, J. W., Subcellular distribution and chemical form of cad- mium in bean plant, Plant Physiology, 1980, 46: 480–482.Google Scholar
- 20.Pathore, V. S., Bajat, Y. P. S, Wittwer, S. H., Subcellular localiza- tion of zinc and calcium in bean (Phaseolus vulgaris L.) tissues, Plant Physiology, 1972, 49: 207–211.Google Scholar
- 21.Liao, X. Y., Chen, T. B., Xie, H. et al., Effect of application of P fertilizer on efficiency of As removal in contaminated soil using phytoremediation: Field demonstration, Acta Scientiae Circumstan- tiae (in Chinese), 2003, 24(3):455–462.Google Scholar
- 24.Liao, X. Y., Xiao, X. Y., Chen, T. B., Effects of Ca and As on As, P and Ca uptake by hyperaccumulator Pteris vittata L. under sand culture, Acta Ecologica Sinica (in Chinese), 2003, 23(10): 2057- 2065.Google Scholar
- 25.Zheng, G. C., Cytobiology (in Chinese), 2nd ed., Beijing: Higher Education Press, 2000: 127.Google Scholar
- 26.Wang, L. J., Liu, Y. L., Vacuoles of plant cells and their physio- logical functions, Plant Physiology Letters (in Chinese), 1998, 34(5): 394–400Google Scholar
- 31.Ernst, W. H. O., Verkleij, J. A. C., Schat, H., Metal tolerance in plants, Acta Botanica Neerlandica, 1992, 41: 229–248.Google Scholar