Abstract
Based on the mass balance equations of solute transfer in the radial chromatographic column, the theoretical expression to describe the column efficiency and shape of elution profile is obtained under linear isotherm case. Moreover, the tendency for the variation of column efficiency and symmetry of peak profile is systematically discussed. The results showed that in radial chromatography the relationship between the column efficiency and volumetric flow rate is similar with that relationship in axial chromatography; relatively high column efficiency still can be obtained under high flow rate in radial chromatography. Accompanying the increase of retention factor of solutes and injection time, the column efficiency decreases monotonously. The effect of column diameter and column length on the column efficiency interfere with each other. It is more advantageous to increase the column efficiency by applying columns with larger column diameter and shorter column length. According to the discussion of the effect of diffusion on the column efficiency, radial chromatography is proved to be suitable for the separation of samples with relatively high diffusion coefficient, which predicts its obvious advantage in the preparative separation of samples such as proteins and DNA.
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References
Sun, T., Chen, G., Liu, Y. et al., Purification of human prothrom- bin from Nitschmann fraction III: using DEAE membrane radial flow chromatography, J. Chromatogr. B, 2000, 742: 109–114.
Liapis, A. I., Theoretical aspects of affinity chromatography, J. Biotechnology, 1989, 11(2/3): 143–160.
Hopf, P., Radial chromatography in industry, Ind. Eng. Chem., 1947, 39: 938–400.
Rice, R. G., Radial flow chromatography, U. S. Patent, 5 433 847, 1995-07-18.
Rice, R. G., Radial flow chromatography, U. S. Patent, 5 484 532, 1996-01-16.
Rice, R. G., Radial flow chromatography, U. S. Patent, 5 589 062, 1996-12-31.
Rice, R.G., Heft, B. K., Radial flow chromatography in compressed Pancake-Shaped Beds, Chem. Eng. Commun., 1990, 98: 231–242.
Rice, R.G., Heft, B. K., Separations via radial flow chromatography in compacted particle beds, AIChE J., 1991, 37(4): 629–632.
Zhang Yukui, Modern Separation and Analysis Methods for Bimolecular, Beijing: Science Press, 2003, 82–119.
Liu Guoquan, Downstream Technology of Bioprocess Engineering, 2nd Edition, Beijing: Chemical Industry Press, 2003, 13th Chapter.
Tharakan, J., Belizaire, M., Ligand efficiency in axial and radial flow immunoaffinity chromatography of factor IX, J. Chromatogr. A, 1995, 702(1/2): 191–196.
Rachinskii, V. V., Basic principles of radial chromatography, J. Chromatogr., 1968, 33: 234–241.
Kalinichev, A. I., Zolotarev, P. P. Methodof moments in the theory of radial-cylindrical frontal dynamics of sorption of substance, Zh. Fiz. Khim., 1977, 51(6): 1478–1479.
Inchin, P. A., Rachinskii, V. V., Theory of radial-cylindrical dynamics of sorption, VI. Action of longitudinal molecular diffusion during filtration to the axis of the sorber, Zh. Fiz. Khim., 1973, 47(7): 1891–1893.
Huang, S. H., Lee, W. C., Tsao, G. T., Mathematical models of radial chromatography, J. Chem. Eng., 1988, 38: l79-l86.
Tsaur, Y., Shallcross, D. C., Comparison of simulated performance of fixed ion-exchange beds in linear and radial flow, Solvent Extr. Ion. Exch., 1997, 15(4): 689–708.
Gu, T. Y., Tsai, G. J., Tsao, G. T., A theoretical study of multicomponent radial flow chromatography, Chem. Eng. Sci., 1991, 46(5-6): 1279–1288.
Munson-McGee, S. H., Fluid dynamics of radial-flow ion-exchange in partially filled columns, Separation Science and Technology, 2000, 35(15): 2415–2429.
Wang Hailin, Master Thesis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 1994.
Li Tong, Ph. D. Thesis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 1994.
Lu Peichang, Dai Chaozheng, Zhang Xiangmin, Basic Theory of Chromatography, 2nd ed., Beijing: Science Press, 1997, Appendix.
Zou Hanfa, Zhang Yukui, Lu Peichang, High-Performance Liquid Chromatography, Beijing: Science Press, 1998, 1–12.
Dai Caozeng, ba]Xiang Zaiyun, Research into character of chromatographic eluting curve by plate theory, Acta Chimica Sinica, 1994, 52(1): 64–70.
Kong Hongwei, Zhang Weibing, Xu Guowang et al., Statistical characteristics of chromatographic profile based on the plate theory, Chinese Journal of Analytical Chemistry, 1999, 27(4): 408- 411.
Yang Changlong, Dalian Institute of Technology, Ph. D. Thesis, 2004.
Gustavsson, P. E., Larsson, P. O., Continuous superporous agarose beds in radial flow columns, J. Chromatogr. A, 2001, 925: 69- 78.
Peter, R. L., Large-scale ion-exchange column chromatography of proteins: Comparison of different formats, J. Chromatogr. B, 2003, 790: 17–33.
Planques, Y., Menozzi, H. P. D., Affinity purification of plasminogen by radial-flow affinity chromatography, J. Chromatogr. A, 1991, 539: 531–533.
Cramer, S. M., Jayaraman, G., Preparative chromatography in biotechnology, Current Opinion in Biotechnology, 1993, 4(2): 217–225.
Gu, T., Tsai, G. J., Tsao, G. T., Multicomponent affinity radial flow chromatography, Separations Technology, 1992, 2(4): 176–182.
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Zhang, W., Shan, Y., Seidel-Morgenster, A. et al. Characteristics of elution profile in radial chromatography under linear conditions. Sc. China Ser. B-Chem. 48, 352–360 (2005). https://doi.org/10.1360/042004-50
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DOI: https://doi.org/10.1360/042004-50