Abstract
The formation of layer silicates on capsuled bacterial cell walls was studied in freshwater microbial mats. The trends associated with Al, Si, Mn and Fe deposits with capsules are consistent with occurrence of layer silicates with 14, 10 and 7 Å X-ray diffraction (XRD) patterns. Scanning electron microscope and transmission electron microscope (SEM and TEM) observations of the microbial mats revealed the presence of microcolonies of rod- and coccus-shaped bacteria with layer-silicate thin films. Field measurements of pH, temperature and Eh indicated that these conditions for bacterial crystallization of layer silicates and hydrated Fe/Mn oxides in freshwaters are as follows: pH 6.3 to 7.8, 12 to 20 °C and Eh −24 to +200 mV. Glass slides kept for 3 weeks in the beakers with natural freshwater and river sediments were coated with brown materials. These materials were identified as layer silicates and colonized bacteria formed under photosynthetic conditions. The well-developed holdfasts on Leptothrix discophora bacterial cells are mainly associated with poorly crystalline layer silicates and hydrated Fe-Mn oxides. Semiquantitative elemental analyses of holdfasts using energy-dispersive X-rays (EDX) indicated that layer-silicate crystallization covers the cell at an early stage. Iron and manganese crystallization develops at a later stage, where aluminum substitution occurs in crystal structures. Laboratory experimental results indicated that layer silicates grew from a biochemical origin, rather than from inorganic origins in freshwater. Layer-silicate formation is linked with bacteria in microbial mats.
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References
Amouric M, Parron C. 1985. Structure and growth mechanisms of glauconite as seen by high resolution transmission electron microscopy. Clays Clay Miner 33:474–482.
Beveridge TJ, Fyfe WS. 1985. Metal fixation by bacterial cell wall. Can J Earth Sci 22:1893–1898.
Beveridge TJ, Murray RGE. 1976. Uptake and retention of metal by cell walls of Bacillus subtilis. J Bacteriol 127:1502–1518.
Burdige DJ, Kepkay PE. 1983. Determination of bacterial Mn oxidation rates in sediments using an in situ dialysis technique. I. Laboratory studies. Geochim Cosmochim Acta 47:1907–1916.
Cairns-Smith AG. 1982. Genetic takeover and the mineral origins of life. London: Cambridge Univ Pr. 477 p.
Chapnick SD, Moore WS, Nealson KH. 1982. Microbiolog-ically mediated manganese oxidation in a fresh water lake. Limnol Oceanogr 27:1004–1014.
Cowen JP, Bruland KW. 1985. Metal deposits associated with bacteria: Implications for Fe and Mn marine biogeo-chemistry. Deep Sea Res 32:253–272.
Cowen JP, Massoth GJ, Baker ET. 1986. Bacterial scavenging of Mn and Fe in a mid- to far-field hydrothermal particle plume. Nature 322:169–171.
Doyle GJ, Marquis RE. 1994. Elastic, flexible peptidoglycan and bacterial cell wall properties.. Trends Microbio 2:57–60.
Ehrlich HL. 1981. Geomicrobiology. New York: Marcel Dekker. 424 p.
Emerson D, Revsbech NP. 1994a. Investigation of an iron-oxidizing microbial mat community located near Aarhus, Denmark: Field studies. Appl Environ Microbiol 60:4022–4031.
Emerson D, Revsbech NP. 1994b. Investigation of an iron-oxidizing microbial mat community located near Aarhus, Denmark: Laboratory studies. Appl Environ Microbiol 60:4032–4038.
Ferris FG, Fyfe WS, Beveridge TJ. 1987. Bacteria as nucleation sites for authigenic minerals in a metal contaminated lake sediment. Chem Geol 63:225–232.
Ferris FG, Fyfe WS, Beveridge TJ. 1988. Metallic ion binding by Bacillus subtilis: Implications for the fossilization of microorganisms. Geology 16:149–152.
Filip Z. 1979. Wechselwirkungen von Mikroorganismen und Tonmineralen—eine Übersicht. Zeitschrift für Pflanzenernährung und Bodenkunde 142:375–386.
Golden DC, Chen CC, Dixon JB, Tokashiki Y. 1988. Pseu-domorphic replacement of manganese oxides by iron oxides. Geoderma 42:199–211.
Hem JD, Lind CJ. 1983. Nonequilibrium models for predicting forms of precipitated manganese oxides. Geochim Cosmochim Acta 47:2037–2046.
Kohler B, Singer A, Stoffers P. 1994. Biogenic nontronite from marine white smoker chimneys. Clays Clay Miner 42:689–701.
Konhauser KO, Fyfe WS, Ferris FG, Beveridge TJ. 1993. Metal sorption and mineral precipitation by bacteria in two Amazonian river systems: Rio Solimoes and Rio Negro. Brazil Geol 21:1103–1106.
Larock PA, Ehrlich HL. 1975. Observations of bacteria microcolonies on the surface of ferromanganese nodules from Blake Plateau by scanning electron microscopy. Microbial Ecol 2:84–96.
Mann H, Fyfe WS. 1984. An experimental study of algal uptake of U, Ba, V, Co and Ni from dilute solutions. Chem Geol 44:385–398.
Mann H, Fyfe WS. 1985. Uranium uptake by algae: Experimental and natural environments. Can J Earth Sci 22:1899–1903.
Mann H, Fyfe WS. 1989. Metal uptake and Fe-, Ti-oxide biomineralization by acidophilic microorganisms in mine-waste environments, Elliot Lake, Canada. Can J Earth Sci 26:2731–2735.
Mann H, Tazaki K, Fyfe WS, Kerrich R. 1992. Microbial accumulation of iron and manganese in different aquatic environments: An electron optical study. In: Skinner HCW, Fitzpatrick RW, editors. Biomineralization; Processes of iron and manganese. Catena Suppl 21:115–131.
Mustoe GE. 1981. Bacterial oxidation of manganese and iron in a modern cold spring. Geol Soc Am Bull Part 1, 92:147–153.
Nealson KH, Tebo B. 1980. Structural features of manganese precipitating bacteria. Origins Life 10:117–126.
Payne SM. 1993. Iron acquisition in microbial pathogenesis. Trends Microbiol 1:66–69.
Pracejus B, Bolton BR. 1992. Interdependence of Mn, Fe, and clay mineral formation on Groote Eylandt, Australia: A model for modern and ancient weathering environments. In: Skinner HCW, Fitzpatrick RW, editors. Biomineralization; Processes of iron and manganese. Catena Suppl 21:371–397.
Robbins EI, D’Agostino JP, Ostwald J, Fanning DS, Carter V, Van Hoven RL. 1992. Manganese nodules and microbial oxidation of manganese in the Huntley Meadows wetland, Virginia, USA. In: Skinner HCW, Fitzpatrick RW, editors. Biomineralization; Processes of iron and manganese. Catena Suppl 21:179–202.
Schindler J. 1993. Dynamics of Bacillus colony growth. Micro Biol 1:333–337.
Schmidt FG, Robbins, EI. 1992. New evidence of an organic contribution to manganese precipitation in iron-formation and review of sedimentary conditions in the Cuyuna north range, Minnesota. In: Skinner HCW, Fitzpatrick RW, editors. Biomineralization; Processes of iron and manganese. Catena Suppl 21:399–419.
Skinner HCW, Fitzpatrick RW. 1992. Iron and manganese biomineralization. In: Skinner HCW, Fitzpatrick RW, editors. Biomineralization; Processes of iron and manganese. Catena Suppl 21:1–6.
Snowball IF. 1994. Bacterial magnetite and the magnetic properties of sediments in a Swedish lake. Earth Planet Sci Lett 126:129–142.
Tanaka A, Seyama H, Soma M. 1994. Iron- and manganese-rich sediments as an indicator of hot spring activities at the bottom of Lake Mashu, Japan. Geochem J 28:289–306.
Tazaki K. 1995. Electron microscopic observation of bio-mineralization in biomats from hot springs. J Geol Soc Jpn 101:304–314.
Tazaki K, Hattori T, Oka M, Iizumi S. 1995. Incipient stages of microbial manganese nodule precipitation in freshwater. J Geol Soc Jpn 101:87–98.
Thamdrup B, Glud RN, Hansen JW. 1994. Manganese oxidation and in situ manganese fluxes from a coastal sediment. Geochim Cosmochim Acta 58:2563–2570.
Tunnicliffe V, Fontaine AR. 1987. Faunal composition and organic surface encrustations at hydrothermal vents on the southern Juan de Fuca Ridge. J Geophys Res 33:303–311.
Usui A, Mita N. 1995. Geochemistry and mineralogy of modern buserite deposit from a hot spring in Hokkaido, Japan. Clays Clay Miner 43:116–127.
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Tazaki, K. Biomineralization of Layer Silicates and Hydrated Fe/Mn Oxides In Microbial Mats: An Electron Microscopical Study. Clays Clay Miner. 45, 203–212 (1997). https://doi.org/10.1346/CCMN.1997.0450208
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DOI: https://doi.org/10.1346/CCMN.1997.0450208