Summary
We developed a new cell stimulation method in which magnetic microparticles (MPs) were introduced into the cytoplasm of cultured myoblasts and the cells were cultured in a magnetic field. The differentiation of myoblasts was examined from the viewpoint of their morphology and myogenin production. After exposure to the magnetic field, the cells containing MPs became larger and were elongated along the axis of the magnetic poles. Myogenin, a muscle-specific regulatory factor involved in controlling myogenesis, was formed earlier, and myotubes were seen earlier and more frequently in this group of myoblasts than in the other groups (cells alone without magnetic field, cells containing MPs but without magnetic field, and cells alone with magnetic field). Moreover, we succeeded in differentiation of early muscle cells with striated myofibrils in culture at 0.05 T. The precisely quantitative and stable stimulus induced by a magnetic field developed in the present study offers a new approach to elucidate the entire process of myoblast differentiation into myotubes.
Similar content being viewed by others
References
Alberts, B.; Bray, D.; Lewis, J., et al. Molecular biology of the cell, 3rd ed. New York: Garland Publishing; 1994:834–847.
Atomi, Y.; Kamoto, M. Responses of muscle cell to mechanical stretch [in Japanese]. Soshiki Baiyo 22:403–407; 1996.
Chomczynski, P.; Sacchi, N. Single-step of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156–159; 1987.
Mitsumoto, Y.; Liu, Z.; Klip, A. A long-lasting vitamin C derivative, ascorbic acid 2-phosphate, increases myogenin gene expression and promotes differentiation in L6 muscle cells. Biochem. Biophys. Res. Commun. 199:394–402; 1994.
Schägger, H.; Jagow, G. von. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368–379; 1987.
Sokabe, M.; Naruse, K. Methods of stretching cultured cells: their advantages and disadvantages [in Japanese]. Soshiki Baiyo 22:413–417; 1996.
Vandenburgh, H. H. A computerized mechanical cell stimulator for tissue culture: effects on skeletal muscle organogenesis. In Vitro Cell. Dev. Biol. 24:609–619; 1988.
Vandenburgh, H. H.; Karlisch, P. Longitudinal growth of skeletal myotubes in vitro in a new horizontal mechanical cell stimulator. In Vitro Cell. Dev. Biol. 25:607–616; 1989.
Vandenburgh, H. H.; Kaufman, S. Stretch-induced growth of skeletal myotubes correlates with activation of the sodium pump. J. Cell Physiol. 109:205–214; 1981.
Vandenburgh, H. H.; Shansky, J.; Karlisch, P., et al. Mechanical stimulation of skeletal muscle generates lipid-related second messengers by phospholipase activation. J. Cell Physiol. 155:63–71; 1993.
Wang, N.; Butler, J. P.; Ingber, D. E. Mechanotransduction across the cell surface and through the cytoskeleton. Science 260:1124–1127; 1993.
Wang, N.; Ingber, D. E. Control of cytoskeletal mechanics by extracellular matrix, cell shape, and mechanical tension. Biophys. J. 66:2181–2189; 1994.
Wang, N.; Ingber, D. E. Probing transmembrane mechanical coupling and cytomechanics using magnetic twisting cytometry. Biochem. Cell Biol. 73:327–335; 1995.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yuge, L., Kataoka, K. Differentiation of myoblasts is accelerated in culture in a magnetic field. In Vitro Cell.Dev.Biol.-Animal 36, 383–386 (2000). https://doi.org/10.1290/1071-2690(2000)036<0383:DOMIAI>2.0.CO;2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1290/1071-2690(2000)036<0383:DOMIAI>2.0.CO;2