Summary
A continuous cell line, PBLE, was developed from the adherent cells in a culture of peripheral blood leukocytes from the American eel, Anguilla rostrata. The cells were grown in Leibovitz's L-15 basal medium supplemented with 20% fetal bovine serum (FBS). Under normal culture conditions at 18° C, the morphology of PBLE was fibroblast-like. The cultures have been subcultured over 80 times and have been cryopreserved successfully. These cells have a diploid karyotype of 38 chromosomes, survived temperatures from 5 to 36° C, and proliferated at temperatures from 5° C to at least 30° C. PBLE underwent apoptosis in response to gliotoxin, but did not show a respiratory burst. Results suggest that PBLE may have arisen from a circulating mesenchymal stem cell. PBLE was susceptible to Chum salmon reovirus (CSV) and supported CSV replication. Therefore this cell line should be useful in studying eel specific virus-host interactions.
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Dewitte-Orr, S.J., Lepic, K., Bryson, S.P. et al. Development of a continuous cell line, PBLE, from an American eel peripheral blood leukocyte preparation. In Vitro Cell.Dev.Biol.-Animal 42, 263–272 (2006). https://doi.org/10.1290/0604023.1
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DOI: https://doi.org/10.1290/0604023.1