Summary
Felal calf serum (FCS) is conventionally used for animal cell cultures due to its inherent growth-promoting activities. However animal welfare issues and stringent requirements for human transplantation studies demand a suitable alternative for FCS. With this view, we studied the effect of FCS, human AB serum (ABS), and human umbilical cord blood serum (UCBS) on murine islets of Langerhans and human bone marrow-derived mesenchymal-like cells (hBMCs). We found that there was no difference in morphology and functionality of mouse islets cultured in any of these three different serum supplements as indicated by insulin immunostaining. A comparative analysis of hBMCs maintained in each of these three different serum supplements demonstrated that UCBS supplemented media better supported proliferation of hBMCs. Moreover, a modification of adipogenic differentiation protocol using UCBS indicates that it can be used as a supplement to support differentiation of hBMCs into adipocytes. Our results demonstrate that UCBS not only is suitable for maintenance of murine pancreatic islets, but also supports attachment, propagation, and differentiation of hBMCs in vitro. We conclude that UCBS can serve as a better serum supplement for growth, maintenance, and differentiation of hBMCs, making it a more suitable supplement in cell systems that have therapeutic potential in human transplantation programs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barria, E.; Mikels, A.; Haas, M. Maintenance and self-renewal of long term reconstituting hematopoietic stem cells supported by amniotic fluid. Stem cells and development. 13:548–562; 2004.
Bhattacharya, A.; Band, A. H., Ramakrishnan, S., Talwar, G. P., et al. Growth and maintenance of mammalian cell lines in human cord serum. Indian Journal of Experimental Biology. 21:99–102; 1983.
Capiaumont, J.; Ostrovidov, S.; Legrand, C.; Belleville, F.; Nabel, P. Bovine whey: a substitute for FBS in CHO-KI cell cultures. In Vitro Cell Dev Biol Anim. 32(1):8–12; 1996.
Deshpande, M.; Katdare, M.; Parab, P. Supplementation with soybean lipids reduces goat serum-induced apoptosis in the B-cell hybridoma CC9C10. In Vitro Cell. Dev. Biol. Anim. (Letter to the Editor). 36:1–3, 2000.
Filipie, B.; Shehata, M.; Toth, S.; Schwarzmeier, J.; Koren, S. Novel serum replacement based on bovine ocular fluid: a useful tool for cultivation of different animal cells in vitro. ALTEX. 19(1):15–20; 2002.
Frassoni F; Repetto M; Piaggio G; Podesta M.; Marmont A. M. Human serum-dependent survival of GM-CFCs in vitro from patients with chronic granulocytic leukemia. Leuk. Res. 11(1):3–6; 1987.
Haynesworth, S. E.; Goshima, J.; Goldberg, V. M.; Caplan, A. I. Characterization of cells with osteogenic potential from human marrow. Bone. 13:81–88; 1992.
Kassem M; Kristiansen M.; Abdallah B. M. Mesenchymal stem cells: cell biology and potential use in therapy. Basic and Clinical Pharmacology and Toxicology 95:209–214; 2004.
Klein, R.; Dumble, L. J. Transmission of Creutzfeldt-Jakob disease by blood transfusion. Lancet. 341:768; 1993.
Koivisto, H.; Hyvarinen, M.; Stromberg, A. M.; Inzunza, J.; Matilainen, E.; Mikkola, M.; Hovatta, O.; Teerijoki, H. Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor. Reprod. Biomed. Online. 9(3):330–337; 2004.
Paranjape, S. Goat serum: an alternative to fetal bovine serum in biomedical research. Indian J. Exp. Biol. 42(1):26–35; 2004.
Pittenger, M. F.; Mackay, A. M.; Beck, S. C., et al. Multilineage potential of adult human mesenchymal stem cells. Science. 284(341):143–147; 1999.
Sasse, M.; Lengwinat, T.; Henklein, P.; Hlinak, A.; Schade, R. Replacement of fetal calf serum in cell cultures by an egg yolk factor with cholecystokinin/gastric-like immunoreactivity, Altern. Lab. Anim. 28(6):815–831; 2000.
Shahdadfar, A.; Fronsdal, K.; Haug, T.; Reiholt, F. P.; Brinchmann, J. E. In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression and transcriptome stability, stem cells 23(9):1357–1366; 2005.
Shewade, Y.; Umarani, M.; Bhonde, R. R. Large-scale isolation of islets by tissue culture of adult mouse pancreas. Transplantation. Proc. 31:1721–1723; 2001.
Stute, N.; Holtz, K.; Bubenheim, M.; Lange, C.; Blake, F.; Zander, A. Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use. Exp. Hematology 32:1212–1225; 2004.
Terasawa, T.; Ito, T.; Matsuda, M.; Azuma, T.; Kasai, S. High erythroid burst-promoting activity in sera from umbilical cord blood. Jpn. J. Physiol. 34(2):217–224; 1984.
Xia, D.; He, H.; Lei, Z.; Zhang, P.; Guo, Y. Effects of human umbilical cord senum on proliferation and insulin content of human fetal islet-like cell clusters. Hepatobiliary Pancreat Dis Int. 3(1):144–148; 2004.
Yamaguchi M., Yamaguchi, M., Hirayama, F.; Wakamoto, S., et al. Bone marrow stromal cells prepared using AB serum and bFGF for hemato-poietic stem cells expansion. Transfusion 42(7):921–927; 2002.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Phadnis, S.M., Joglekar, M.V., Venkateshan, V. et al. Human umbilical cord blood serum promotes growth, proliferation, as well as differentiation of human bone marrow-derived progenitor cells. In Vitro Cell.Dev.Biol.-Animal 42, 283–286 (2006). https://doi.org/10.1290/0512087.1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1290/0512087.1