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Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features

  • Chisato Shimizu
  • Hiroko Shike
  • Denise M. Malicki
  • Eric Breisch
  • Mark Westerman
  • John Buchanan
  • Heather R. Ligman
  • Ruth B. Phillips
  • James M. Carlberg
  • Jack Van Olst
  • Jane C. Burns
Articles Cell and Tissue Models

Summary

The genus Morone is an important one for U.S. aquaculture, but there has been no available cell line from this genus. We report here a cell line (the WBE line) derived from white bass embryos that has been grown for more than 80 passages over 21 mo in Dulbecco modified Eagle medium supplemented with fetal bovine serum. The WBE line showed epithelial features with positive immunohistochemical staining for cytokeratin and intercellular junctions when observed by electron microscopy. The doubling time, transformation characteristics, response to cryopreservation, and karyotype were determined. The WBE line was also tested by polymerase chain, reaction to verify the species of origin and to screen for mycoplasma infection. This epithelial cell line may prove useful for the study of host-pathogen interactions that occur at the epithelial surface in this commercially important fish species.

Key words

teleost electron microscopy cytokeratin staining fish karyotype 

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Copyright information

© Society for In Vitro Biology 2003

Authors and Affiliations

  • Chisato Shimizu
    • 6
  • Hiroko Shike
    • 6
  • Denise M. Malicki
    • 1
    • 3
  • Eric Breisch
    • 2
    • 3
  • Mark Westerman
    • 4
  • John Buchanan
    • 4
  • Heather R. Ligman
    • 5
  • Ruth B. Phillips
    • 5
  • James M. Carlberg
    • 3
    • 4
  • Jack Van Olst
    • 4
  • Jane C. Burns
    • 6
  1. 1.Department of PathologyUCSD School of Medicine, University of California, San DiegoLa Jolla
  2. 2.Department of SurgeryUCSD School of Medicine, University of California, San DiegoLa Jolla
  3. 3.San Diego Children's HospitalSan Diego
  4. 4.Kent SeaTech CorporationSan Diego
  5. 5.Biological ScienceWashington State University VancouverVancouver
  6. 6.Department of Pediatrics, UCSD School of MedicineUniversity of California, San DiegoLa Jolla

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