Skip to main content

Advertisement

Log in

Effect of snack eating on sensitive salivary stress markers cortisol and chromogranin A

  • Short Communication
  • Published:
Environmental Health and Preventive Medicine Aims and scope

Abstract

Objectives

To investigate the effect of snack eating on salivary cortisol and chromogranin A (CgA).

Methods

From 14∶00 to 18∶00, starting two hours after consumption of a midday meal, saliva samples were collected every 30 minutes from 15 healthy males, 7 of whom (snack group) ate a snack immediately after the sampling at 15∶00. Salivary cortisol and CgA levels were determined by ELISA. Samples were controlled according to salivary flow rates.

Results

For the snack group, after snack consumption, salivary cortisol increased to exceed significance (p<0.05) at 15∶30 and rose even higher at 16∶00. In the control group, there was no such change. There was no significant change in salivary CgA in either the snack group or the control groups during the sampling period.

Conclusions

These findings suggest that no food should be consumed for at least 90 mins before saliva sampling for cortisol determination and that salivary CgA is probably not affected by snack eating.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Krischbaum C, Hellhammer DH. Salivary cortisol in psychoneuroendocrine research: recent developments and applications. Psychoneuroendocrinology 1994; 19: 313–333.

    Article  Google Scholar 

  2. Smith AD, Winkler H. Purilication and properties of an acidic protein from chromaffin granules of bovine adrenal medulla. Biochem. J. 1967; 103: 483–492.

    PubMed  CAS  Google Scholar 

  3. Smith WJ, Kirshner N. A specific soluble protein from the catecholamine storage vesicles of bovine adrenal medulla. Molec. Pharmacol. 1967; 3: 52–62.

    CAS  Google Scholar 

  4. Winkler H, Fischer-Colbrie R. The chromogranins A and B: the first 25 years and future perspectives. Neuroscience 1992; 49: 497–528.

    Article  PubMed  CAS  Google Scholar 

  5. Nakane H, Asami O, Yamada Y, Harada T, Matsui N, Kanno T, Yanaihara N. Salivary chromogranin A as an index of psychosomatic stress response. Biomed. Res. 1998; 19: 401–406.

    CAS  Google Scholar 

  6. Goldberg DP. Manual of the General Health Questionnaire. Windsor; NFER Publishing, 1978.

    Google Scholar 

  7. Zung WWK. A self-rating depression scale. Arch. Gen. Psychiatry 1965; 12: 63–70.

    PubMed  CAS  Google Scholar 

  8. Fukuda K, Kobayashi S. The Japanese version of the selfrating depression scale (in Japanese). Kyoto: Sankyobo, 1983.

    Google Scholar 

  9. Morimoto K. Lifestyle and health. Jpn. J. Hyg. 2000; 54: 572–591.

    CAS  Google Scholar 

  10. Hagihara A, Morimoto K. Personal health practices and attitudes toward nonsmokers’ legal rights in Japan. Soc. Sci. Med. 1991; 33: 717–721.

    Article  PubMed  CAS  Google Scholar 

  11. Shimada M, Takahashi K, Ohkawa T, Segawa M, Higurashi M. Determination of salivary cortisol by ELISA and its application to the assessment of the circadian rhythm in children. Horm. Res. 1995; 44: 213–217.

    Article  PubMed  CAS  Google Scholar 

  12. Nagasawa S, Nishikawa Y, Jun L, Futai Y, Kanno T, Iguchi K, Mochizuki T, Hoshino M, Yanaihara C, Yanaihara N. Simple enzyme immunoassay for the measurement of immunoreactive chromogranin A in human plasma, urine and saliva. Biomed. Res. 1998; 19: 407–410.

    CAS  Google Scholar 

  13. Nakamura Y, Morimoto T. Basic physiology for dental students (in Japanese). Tokyo: Ishiyaku Publishers. 1987.

    Google Scholar 

  14. Toda M, Morimoto K, Fukuda S, Hayakawa K. Lifestyle, mental health status and salivary secretion rates. Environ. Health Prev. Med. 2002; 6: 260–263.

    Article  Google Scholar 

  15. Quigley ME, Yen SSC. A mid-day surge in cortisol levels. J. Clin. Endocrinol. Metab. 1979; 49: 945–947.

    Article  PubMed  CAS  Google Scholar 

  16. Knoll E, Müller FW, Ratge D, Bauersfeld W, Wisser H. Influence of food intake on concentrations of plasma catecholamines and cortisol. J. Clin. Chem. Clin. Biochem. 1984; 22: 597–602.

    PubMed  CAS  Google Scholar 

  17. Fukuda S, Morimoto K. Lifestyle, stress and cortisol response: review II-lifestyle.-Environ. Helath Prev. Med. 2001; 6: 15–21.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kanehisa Morimoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Toda, M., Morimoto, K., Nagasawa, S. et al. Effect of snack eating on sensitive salivary stress markers cortisol and chromogranin A. Environ Health Prev Med 9, 27–29 (2004). https://doi.org/10.1265/ehpm.9.27

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1265/ehpm.9.27

Key words

Navigation