Biological Procedures Online

, Volume 6, Issue 1, pp 61–66 | Cite as

Serum-free cryopreservation of five mammalian cell lines in either a pelleted or suspended state

Open Access


Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative. Using five mammalian lines (Crandell Feline Kidney, MCF7, A72, WI 38 and NB324K), we found that the serum-free alternative preserves nearly as efficiently as the serum-containing preservatives. Second, we compared cryostorage of those cells in suspended versus a pellet form using both aforementioned cryopreservatives. Under our conditions, cells were in general recovered equally well in a suspended versus a pellet form.

Indexing terms

Cryopreservation Cells, Cultured Culture Media, Serum-Free 


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Copyright information

© Springer 2004

Authors and Affiliations

  1. 1.Biology DepartmentChadron State CollegeChadron
  2. 2.Mathematics DepartmentChadron State CollegeChadron

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