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Synthesis by high-efficiency liquid-phase (HELP) method of oligonucleotides conjugated with high-molecular weight polyethylene glycols (PEGs)

Biological Procedures Online volume 1, pages 59–69 (1998)Cite this article

Abstract

The chemical modification of synthetic oligonucleotides has recently been investigated to improve their pharmacological utilization. In addition to chemical alterations of the backbone and of the heterocyclic bases, their conjugation with amphiphylic moieties, such as the polyethylene glycol has been proposed. The large scale production of these molecules as demanded for commercial purposes is hampered by the heterogeneity of the solid-phase processes and by the low reactivity of high-molecular weight PEGs in solution. A new synthetic procedure based on the recently developed liquid-phase method (HELP), has been set up to overcome these limitations.

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Author notes

  1. G. M. Bonora

    Present address: Dept. of Chemical Sciences, University of Trieste, Via Giorgieri 1, 34127, Trieste, Italy

Affiliations

  1. Pharmaco-Chemico-Technological Department, University of Cagliari, Via Ospedale 72, 09127, Cagliari, Italy

    G. M. Bonora

  2. University of Padova, Italy

    S. Zaramella & F. M. Veronese

Authors
  1. G. M. Bonora
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  2. S. Zaramella
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  3. F. M. Veronese
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Correspondence to G. M. Bonora.

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Bonora, G.M., Zaramella, S. & Veronese, F.M. Synthesis by high-efficiency liquid-phase (HELP) method of oligonucleotides conjugated with high-molecular weight polyethylene glycols (PEGs). Biol Proced Online 1, 59–69 (1998). https://doi.org/10.1251/bpo2

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  • DOI: https://doi.org/10.1251/bpo2

Keywords

  • Cholic Acid
  • Sterile Filter
  • Biological Procedure
  • Biological Procedure Online
  • Eluent Acetone