Abstract
DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division. DNMT1 expression is tightly regulated within the cell cycle. Our previous study showed that the binding of a protein with an apparent size of ∼40 kDa on DNMT1 3′-UTR triggered the destabilization of DNMT1 mRNA transcript during Go/G1 phase. Using RNA affinity capture with the 3′-UTR of DNMT1 mRNA and matrix- assisted laser desorption-time of flight tandem mass spectrometry (MALDI-TOF-MS-MS) analysis, we isolated and identified AUF 1 (AU-rich element ARE:poly-(U)-binding/degradation factor) as the binding protein. We then validated the role of this protein in the destabilization of DNMT1 mRNA. In this report, we detail the different approaches used for the isolation, the identification of a RNA binding protein and the validation of its role.
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Abbreviations
- AUF1:
-
AU-rich element ARE:poly-(U)-binding/degradation factor
- DNMT1:
-
DNA methyltransferase 1
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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Unterberger, A., Torrisani, J. & Szyf, M. A method for purification, identification and validation of DNMT1 mRNA binding proteins. Biol. Proced. Online 10, 47–57 (2008). https://doi.org/10.1251/bpo142
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DOI: https://doi.org/10.1251/bpo142