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Comparative Study of Indocyanine Green Fluorescence Imaging in Lung Cancer with Near-Infrared-I/II Windows

  • Thoracic Oncology
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

We compare the application of intravenous indocyanine green (ICG) fluorescence imaging in lung cancer with near-infrared-I (NIR-I) and near-infrared-II (NIR-II) windows.

Methods

From March to December 2022, we enrolled patients who received an intravenous injection of ICG (5 mg/kg) 1 day before the planned lung cancer surgery. The lung cancer nodules were imaged by NIR-I/II fluorescence imaging systems, and the tumor-to-normal-tissue ratio (TNR) was calculated. In addition, the fluorescence intensity and signal-to-background ratio (SBR) of capillary glass tubes containing ICG covered with different thicknesses of lung tissue were measured by NIR-I/II fluorescence imaging systems.

Results

In this study, 102 patients were enrolled, and the mean age was 59.9 ± 9.2 years. A total of 96 (94.1%) and 98 (96.1%) lung nodules were successfully imaged with NIR-I and NIR-II fluorescence, and the TNR of NIR-II was significantly higher than that of NIR-I (3.9 ± 1.3 versus 2.4 ± 0.6, P < 0.001). In multiple linear regression, solid nodules (P < 0.001) and squamous cell carcinoma (P < 0.001) were independent predictors of a higher TNR of NIR-I/II. When capillary glass tubes were covered with lung tissue whose thickness was more than 2 mm, the fluorescence intensity and the SBR of NIR-II were significantly higher than those of NIR-I.

Conclusions

We verified the feasibility of NIR-II fluorescence imaging in intravenous ICG lung cancer imaging for the first time. NIR-II fluorescence can improve the TNR and penetration depth of lung cancer with promising clinical prospects.

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Acknowledgment

This study was funded by National Natural Science Foundation of China (82003316, 92059203, 92059207, 62027901, 81930053, 81227901), CAS Youth Interdisciplinary Team (JCTD-2021-08), and Fundamental Research Funds for the Central Universities (JKF-YG-22-B005).

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Author notes

  1. Jiahui Mi, Changjian Li, Feng Yang and Xiaojing Shi have contributed equally to this work.

Authors and Affiliations

  1. Department of Thoracic Surgery, Peking University People’s Hospital, Beijing, China

    Jiahui Mi MD, Feng Yang MD, Guanchao Jiang MD, Yun Li MD, Jun Wang MD, Fan Yang MD & Jian Zhou MD

  2. School of Engineering Medicine, Beihang University, Beijing, China

    Changjian Li PhD, Zeyu Zhang PhD & Lishuang Guo PhD

  3. Key Laboratory of Big Data-Based Precision Medicine (Beihang University), Ministry of Industry and Information Technology, Beijing, China

    Changjian Li PhD, Zeyu Zhang PhD & Lishuang Guo PhD

  4. CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing, China

    Xiaojing Shi PhD & Zhenhua Hu PhD

  5. School of Artificial Intelligence, University of Chinese Academy of Sciences, Beijing, China

    Xiaojing Shi PhD & Zhenhua Hu PhD

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  1. Jiahui Mi MD
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Corresponding authors

Correspondence to Fan Yang MD, Zhenhua Hu PhD or Jian Zhou MD.

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Mi, J., Li, C., Yang, F. et al. Comparative Study of Indocyanine Green Fluorescence Imaging in Lung Cancer with Near-Infrared-I/II Windows. Ann Surg Oncol 31, 2451–2460 (2024). https://doi.org/10.1245/s10434-023-14677-9

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  • DOI: https://doi.org/10.1245/s10434-023-14677-9

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