Annals of Surgical Oncology

, Volume 25, Issue 3, pp 737–744 | Cite as

Oncological Assessment of Stent Placement for Obstructive Colorectal Cancer from Circulating Cell-Free DNA and Circulating Tumor DNA Dynamics

  • Goro Takahashi
  • Takeshi Yamada
  • Takuma Iwai
  • Kohki Takeda
  • Michihiro Koizumi
  • Seiichi Shinji
  • Eiji Uchida
Colorectal Cancer

Abstract

Background

The self-expanding metallic stent (SEMS) provides effective decompression for patients with malignant large bowel obstruction (MLBO); however, mechanical damage to malignant cells from insertion may negatively affect prognosis, similar to surgical manipulation, and its oncological safety is unclear. We examined mechanical damage from SEMS placement using circulating cell-free DNA (cfDNA) and circulating tumor DNA (ctDNA).

Methods

Between 1 November 2014 and 30 June 2017, 35 MLBO patients were analyzed, comprising 25 SEMS patients and 10 transanal decompression tube (TDT) patients (control). Blood samples were collected before and after decompression on days 0, 1, 3, and 7. cfDNA, ctDNA, white blood cells, C-reactive protein, and lactate dehydrogenase were analyzed.

Results

The clinical success rates of SEMS and TDT were 88 and 90%, respectively (p = 1.0). The cfDNA concentration on day 7 was significantly higher in the SEMS group than in the TDT group (992 vs. 308 ng/mL; p = 0.005). A significant increase in ctDNA was observed in the SEMS group compared with the TDT group (83% vs. 22%; p = 0.002). The cfDNA concentration showed strong positive correlations with ctDNA and lactate dehydrogenase (R 2 = 0.838 and 0.593, respectively), and a weak positive correlation with C-reactive protein (R 2 = 0.263).

Conclusions

Despite equivalent clinical success rates, SEMS placement increased plasma levels of cfDNA and ctDNA by tumor manipulation, but TDT did not. Colonic stenting showed oncological risk in terms of molecular analysis.

Notes

Acknowledgement

The authors thank Ms. Hazuki Kanno for technical support in the experiments, and Toshiaki Otsuka for statistical advice.

Funding

This research was supported by the Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Nippon Medical School, Tokyo, Japan. The funding source played no role in this study.

Disclosures

Goro Takahashi, Takeshi Yamada, Takuma Iwai, Kohki Takeda, Michihiro Koizumi, Seiichi Shinji, Eiji Uchida have no conflicts of interest to declare.

Supplementary material

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Copyright information

© Society of Surgical Oncology 2017

Authors and Affiliations

  • Goro Takahashi
    • 1
  • Takeshi Yamada
    • 1
  • Takuma Iwai
    • 1
  • Kohki Takeda
    • 1
  • Michihiro Koizumi
    • 1
  • Seiichi Shinji
    • 1
  • Eiji Uchida
    • 1
  1. 1.Department of Gastrointestinal and Hepato-Biliary-Pancreatic SurgeryNippon Medical SchoolTokyoJapan

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