Skip to main content

Advertisement

SpringerLink
Log in

Gain of Function of Mutant TP53 in Glioblastoma: Prognosis and Response to Temozolomide

  • Neuro-Oncology
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Our aim was to investigate the relationship between mutant p53 and the prognosis of malignant glioma treated with temozolomide, and the regulation of mutant TP53 induced drug resistance, by molecular experimentation and a clinical trial.

Methods

Adult patients with newly surgical diagnosed glioblastoma were randomly assigned to receive either temozolomide or semustine after radiation treatment. The statuses of TP53 and expression of TP53 and O6-methylguanine DNA-methyltransferase (MGMT) were determined retrospectively in tumor tissue from enrolled patients. The primary end point was overall survival. Synthetic small interfering RNA was used to knock down mutant TP53 in T98G and U138 cells, which are human glioblastoma cells with a P53 mutation, by screening of exons 4–8. Viable cell survival was measured when these cells were exposed to temozolomide or semustine. Expression of MGMT at the messenger RNA level was also determined.

Results

The overall survival was 34.3 % at 2 years, 22.9 % at 3 years, 11.4 % at 4 years, and 8.6 % at 5 years with temozolomide, versus 18.2, 12.1, 3.0, and 0 %, respectively, with semustine. TP53 mutation and expression of mutant TP53 and MGMT showed significant inverse correlations with overall survival. Knockdown of mutant TP53 led to a fivefold increase in chemosensitivity to temozolomide but not semustine. Mutant TP53 knockdown induced down-regulation of MGMT expression.

Conclusions

Mutant TP53 is strongly associated with a poor prognosis for overall survival in patients with glioblastoma. Also, TP53 mutation may decrease the chemosensitivity of glioblastoma to temozolomide by increasing MGMT expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
FIG. 3

Similar content being viewed by others

References

  1. Wu J, Zhang J, Zhuang D, et al. Current status of cerebral glioma surgery in China. Chin Med J. 2011;124:2569–77.

    PubMed  Google Scholar 

  2. Stupp R, Hegi ME, Mason WP, et al. Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase III study: 5-year analysis of the EORTC-NCIC trial. Lancet Oncol. 2009;10:459–66.

    Article  CAS  PubMed  Google Scholar 

  3. Hegi ME, Diserens AC, Gorlia T, et al. MGMT gene silencing and benefit from temozolomide in glioblastoma. N Engl J Med. 2005;352:997–1003.

    Article  CAS  PubMed  Google Scholar 

  4. Rolhion C, Penault-Llorca F, Kemeny JL, et al. O(6)-methylguanine-DNA methyltransferase gene (MGMT) expression in human glioblastomas in relation to patient characteristics and p53 accumulation. Int J Cancer. 1999;84:416–20.

    Article  CAS  PubMed  Google Scholar 

  5. Blough MD, Zlatescu MC, Cairncross JG. 6O-methylguanine-DNA methyltransferase regulation by p53 in astrocytic cells. Cancer Res. 2007;67:580–4.

    Article  CAS  PubMed  Google Scholar 

  6. Bocangel D, Sengupta S, Mitra S, et al. p53-Mediated down-regulation of the human DNA repair gene O 6-methylguanine-DNA methyltransferase (MGMT) via interaction with Sp1 transcription factor. Anticancer Res. 2009;29:3741–50.

    CAS  PubMed Central  PubMed  Google Scholar 

  7. Louis DN, von Deimling A, Chung RY, et al. Comparative study of p53 gene and protein alterations in human astrocytic tumors. J Neuropathol Exp Neurol. 1993;52:31–8.

    Article  CAS  PubMed  Google Scholar 

  8. Deppert W. Mutant TP53 from guardian to fallen angel? Oncogene. 2007;26:2142–4.

    Article  CAS  PubMed  Google Scholar 

  9. Bossi G, Lapi E, Strano S, et al. Mutant TP53 gain of function: reduction of tumor malignancy of human cancer cell lines through abrogation of mutant TP53 expression. Oncogene. 2006;25:304–9.

    CAS  PubMed  Google Scholar 

  10. Tsang WP, Ho FY, Fung KP, et al. TP53-175H mutant gains new function in regulation of doxorubicin-induced apoptosis. Int J Cancer. 2005;114:331–6.

    Article  CAS  PubMed  Google Scholar 

  11. Halevy O, Michalovitz D, Oren M. Different tumor-derived TP53 mutant exhibit distinct biological activities. Science. 1990;250:113–6.

    Article  CAS  PubMed  Google Scholar 

  12. Selivanova G, Wiman KG. Reactivation of mutant TP53: molecular mechanisms and therapeutic potential. Oncogene. 2007;26:2243–54.

    Article  CAS  PubMed  Google Scholar 

  13. Anker L, Ohgaki H, Ludeke BI, et al. p53 protein accumulation and gene mutations in human glioma cell lines. Int J Cancer. 1993;55:982–7.

    Article  CAS  PubMed  Google Scholar 

  14. Newcomb EW, Madonia WJ, Pisharody S, et al. A correlative study of p53 protein alteration and p53 gene mutation in glioblastoma multiforme. Brain Pathol. 1993;3:229–35.

    Article  CAS  PubMed  Google Scholar 

  15. Koga H, Zhang S, Kumanishi T, et al. Analysis of p53 gene mutations in low-and high-grade astrocytomas by polymerase chain reaction-assisted single-strand conformation polymorphism and immunohistochemistry. Acta Neuropathol. 1994;87:225–32.

    Article  CAS  PubMed  Google Scholar 

  16. Pardo FS, Hsu DW, Zeheb R, et al. Mutant, wild type, or overall p53 expression: freedom from clinical progression in tumours of astrocytic lineage. Br J Cancer. 2004;91:1678–86.

    CAS  PubMed Central  PubMed  Google Scholar 

  17. Petitjean A, Achatz MIW, Borresen-Dale AL, et al. TP53 mutations in human cancers: functional selection and impact on cancer prognosis and outcomes. Oncogene. 2007;26:2157–65.

    Article  CAS  PubMed  Google Scholar 

  18. Watanabe T, Katayama Y, Komine C, et al. O 6-methylguanine-DNA methyltransferase methylation and TP53 mutation in malignant astrocytomas and their relationships with clinical course. Int J Cancer. 2005;113:581–7.

    Article  CAS  PubMed  Google Scholar 

  19. Olivier M, Langerod A, Carrieri P, et al. The clinical value of somatic TP53 gene mutations in 1,794 patients with breast cancer. Clin Cancer Res. 2006;12:1157–67.

    Article  CAS  PubMed  Google Scholar 

  20. Petitjean A, Mathe E, Kato S, et al. Impact of mutant TP53 functional properties on TP53 mutation patterns and tumor phenotype: lessons from recent developments in the IARC TP53 database. Hum Mutat. 2007;28:622–9.

    Article  CAS  PubMed  Google Scholar 

  21. Powell B, Soong R, Iacopetta B, et al. Prognostic significance of mutations to different structural and functional regions of the p53 gene in breast cancer. Clin Cancer Res. 2000;6:443–51.

    CAS  PubMed  Google Scholar 

  22. Peraud A, Kreth FW, Wiestler OD, et al. Prognostic impact of TP53 mutations and P53 protein overexpression in supratentorial WHO grade II astrocytomas and oligoastrocytomas. Clin Cancer Res. 2002;8:1117–24.

    CAS  PubMed  Google Scholar 

  23. Groenendijk FH, Taal W, Dubbink HJ, et al. MGMT promoter hypermethylation is a frequent, early, and consistent event in astrocytoma progression, and not correlated with TP53 mutation. J Neurooncol. 2011;101:405–17.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

Schering Plough provided temozolomide to all enrolled patients free of charge. This work was supported in part by the National Natural Science Foundation of China (30873007).

Disclosure

The authors report no conflict of interest concerning the materials or methods used in this study or the findings specified in this article.

Author information

Authors and Affiliations

  1. Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, China

    Xiang Wang MD, PhD, Chao You MD, Yan-hui Liu MD, PhD & Qing Mao MD

  2. Department of Radiology, Sichuan Cancer Hospital, Chengdu, China

    Jin-xiu Chen MD

  3. Department of Neurosurgery, Sichuan Provincial People’s Hospital, Chengdu, China

    Jin-ping Liu MD

Authors
  1. Xiang Wang MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jin-xiu Chen MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin-ping Liu MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chao You MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yan-hui Liu MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qing Mao MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiang Wang MD, PhD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, X., Chen, Jx., Liu, Jp. et al. Gain of Function of Mutant TP53 in Glioblastoma: Prognosis and Response to Temozolomide. Ann Surg Oncol 21, 1337–1344 (2014). https://doi.org/10.1245/s10434-013-3380-0

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1245/s10434-013-3380-0

Keywords

Search

Navigation