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Intraoperative Molecular Assessment for Lymph Node Metastasis in Head and Neck Squamous Cell Carcinoma Using One-Step Nucleic Acid Amplification (OSNA) Assay

  • Head and Neck Oncology
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

Conventional intraoperative pathological examination for Sentinel node navigation surgery (SNNS) has been controversial. We evaluated the efficacy of one-step nucleic acid amplification (OSNA) assay for intraoperative diagnosis of cervical lymph node (CLN) metastasis compared with histopathological examination in patients with head and neck squamous cell carcinoma (HNSCC).

Methods

A total of 175 CLNs dissected from 56 patients with HNSCC who underwent surgery at Aichi Cancer Center, Kyorin University, Gunma University or Fukushima Medical University, between April 2008 and December 2011 were enrolled. CLN samples were sectioned into four equal pieces, with two of each used for OSNA assay and other histopathological examinations. The diagnostic value of OSNA assay in HNSCC patients in predicting the results of histopathological diagnosis was evaluated using the area under the receiver operating characteristic (AUROC) curve.

Results

OSNA assay showed acceptable efficacy in the detection of pathological CLN metastasis (AUROC 0.918, 95 % confidence interval [CI] 0.852–0.984). Regarding the CK19mRNA cutoff value, the optimum cutoff point in HNSCC patients was 131 copies/μl (sensitivity: 82.4, 95 % CI 65.5–93.2; specificity: 99.3, 95 % CI 96.1–100.0; positive likelihood ratio 116.1; negative likelihood ratio 0.2].

Conclusions

We demonstrated that OSNA assay is useful in intraoperative diagnosis for CLN metastasis in patients with HNSCC. OSNA assay could be applied for SNNS in HNSCC patients.

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Acknowledgment

This research was supported by Grants-in-aid for Scientific Research (C) from the Japan Society for the Promotion of Science, and a Health and Labor Sciences Research Grant for Clinical Cancer Research (H21-Gannrinshou-Ippan-016) from the Ministry of Health, Labor and Welfare, Japan.

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Authors and Affiliations

  1. Department of Otolaryngology, Fukushima Medical University School of Medicine, Fukushima, Japan

    Takashi Matsuzuka MD, PhD, Masahiro Suzuki MD, Tomohiro Miura MD, PhD & Koichi Omori MD, PhD

  2. Department of Otolaryngology-Head and Neck Surgery, Gunma University Graduate School of Medicine, Maebashi, Japan

    Katsumasa Takahashi MD, PhD & Nobuhiko Furuya MD, PhD

  3. Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Japan

    Daisuke Kawakita MD, PhD & Keitaro Matsuo MD, PhD, MSc

  4. Department of Otolaryngology-Head and Neck Surgery, Kyorin University School of Medicine, Mitaka, Japan

    Naoyuki Kohno MD, PhD, Hiroshi Nagafuji MD & Koichi Yamauchi MD, PhD

  5. Department of Pathology and Molecular Diagnosis, Aichi Cancer Center Hospital, Nagoya, Japan

    Yasushi Yatabe MD, PhD

  6. Department of Head and Neck Surgery, Aichi Cancer Center Hospital, Nagoya, Japan

    Yasuhisa Hasegawa MD, PhD

Authors
  1. Takashi Matsuzuka MD, PhD
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  2. Katsumasa Takahashi MD, PhD
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  3. Daisuke Kawakita MD, PhD
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  4. Naoyuki Kohno MD, PhD
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  5. Hiroshi Nagafuji MD
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  6. Koichi Yamauchi MD, PhD
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  7. Masahiro Suzuki MD
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  8. Tomohiro Miura MD, PhD
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  9. Nobuhiko Furuya MD, PhD
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  10. Yasushi Yatabe MD, PhD
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  11. Keitaro Matsuo MD, PhD, MSc
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  12. Koichi Omori MD, PhD
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  13. Yasuhisa Hasegawa MD, PhD
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Corresponding author

Correspondence to Yasuhisa Hasegawa MD, PhD.

Additional information

Takashi Matsuzuka, Katsumasa Takahashi, Daisuke Kawakita and Naoyuki Kohno contributed equally to this work, and all should be considered first author.

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Matsuzuka, T., Takahashi, K., Kawakita, D. et al. Intraoperative Molecular Assessment for Lymph Node Metastasis in Head and Neck Squamous Cell Carcinoma Using One-Step Nucleic Acid Amplification (OSNA) Assay. Ann Surg Oncol 19, 3865–3870 (2012). https://doi.org/10.1245/s10434-012-2409-0

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  • DOI: https://doi.org/10.1245/s10434-012-2409-0

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