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Overexpressions of RACK1 and CD147 Associated with Poor Prognosis in Stage T1 Pulmonary Adenocarcinoma

  • Translational Research and Biomarkers
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Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

RACK1 has been shown to be able to interact with some key cellular proteins involved in tumor development and progression. Our study showed that the expressions of RACK1 and CD147 are correlated with each other. The purpose of this study is to clarify the relationship between expression of RACK1 and CD147 in 180 patients with operable stage T1 human pulmonary adenocarcinoma and their clinicopathological features and prognostic significance.

Methods

DNA transfection and RNA interference of RACK1 were conducted to produce pulmonary adenocarcinoma cell lines with differential RACK1 expression. Western blot and RT-PCR were used to quantify RACK1 and CD147 expression in protein and mRNA levels in pulmonary adenocarcinoma cell lines. Immunohistochemistry, double-labeling immunofluorescence, confocal laser scanning microscopy, and Western blot were used to correlate the clinicopathological significance of RACK1 and CD147 expression in cases of stage T1 pulmonary adenocarcinoma.

Results

We detected high levels of RACK1 and CD147 mRNA as well as protein expression in pulmonary adenocarcinoma in vitro. In pulmonary adenocarcinoma, the expression of RACK1 and CD147 were correlated both in vitro and in vivo. Our clinicopathological analysis demonstrated that RACK1 or CD147 expression correlated with higher incidence of lymph node metastasis and lower differentiation than tumors that were negative for expression of either RACK1 or CD147. The expression of RACK1 and CD147 was not associated with the patient age or gender. Multivariate analysis demonstrated that the co-overexpression of RACK1 and CD147 was an independent prognostic factor for stage T1 pulmonary adenocarcinoma (P = 0.012).

Conclusions

Tumor invasiveness is associated with expression of RACK1 and CD147 in pulmonary adenocarcinoma. The co-expression of RACK1 and CD147 could be an important prognostic biomarker for stage T1 pulmonary adenocarcinoma.

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Acknowledgment

This study was supported by National Nature Science Foundation of China (No. 30971114).

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Authors and Affiliations

  1. Department of Thoracic Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China

    Xinwen Zhong & Lin Zhang Ph.D

  2. Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China

    Mo Li, Fei Wu, Enhua Wang Ph.D & Yuchen Han Ph.D

  3. Department of Critical Care Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China

    Beibei Nie

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  1. Xinwen Zhong
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  2. Mo Li
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  3. Beibei Nie
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  4. Fei Wu
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  5. Lin Zhang Ph.D
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  6. Enhua Wang Ph.D
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  7. Yuchen Han Ph.D
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Correspondence to Yuchen Han Ph.D.

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Zhong, X., Li, M., Nie, B. et al. Overexpressions of RACK1 and CD147 Associated with Poor Prognosis in Stage T1 Pulmonary Adenocarcinoma. Ann Surg Oncol 20, 1044–1052 (2013). https://doi.org/10.1245/s10434-012-2377-4

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  • DOI: https://doi.org/10.1245/s10434-012-2377-4

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