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Inverse Association between miR-194 Expression and Tumor Invasion in Gastric Cancer

Annals of Surgical Oncology volume 19, pages 509–517 (2012)Cite this article

Abstract

Background

MiR-194 has been shown to be specifically expressed in the human gastrointestinal tract and may play an antimetastatic role in primary liver cancer cells. However, the role of miR-194 in gastric cancer is still unclear.

Methods

Total RNA was extracted from tissues of 119 patients with gastric cancer and three gastric cancer cell lines (SGC-7901, MGC-803, and BGC-823). Expression levels of miR-194 were determined by real-time polymerase chain reaction (PCR). Moreover, a MTT proliferation assay and transwell cell invasion assay were performed to study the effect of miR-194 on SGC-7901 cell proliferation and invasion. Finally, we used real-time PCR and western blot to verify which gene was the target of miR-194 in gastric cancer.

Results

Though there was no significant difference between cancerous and matching noncancerous tissues, we found patients with lower expression of miR-194 tended to have larger tumor size (P = 0.002) and more advanced pT stage (P = 0.028) in gastric cancer. Moreover, the expression of miR-194 was significantly lower in Borrmann IV type gastric cancer than in Borrmann I, II, and III types (P = 0.019). Furthermore, an in vitro invasion assay indicated that the penetrated cell intensity after miR-194 mimics transfection was significantly lower than the control. However, overexpression of miR-194 had little effect on the SGC-7901 cell cycle and proliferation. The results of real-time PCR and western blot highlighted that miR-194 interacted with N-cadherin and negatively regulated its expression at the translational level.

Conclusion

These findings imply that miR-194 might play an important role in gastric cancer invasion and progression.

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Acknowledgment

This work was supported by National Science Foundation of China (No. 30972879 and No. 81000943), Specialized Research Fund for the Doctoral Program of Higher Education (No. 200801590006), and Natural Science Foundation of Liaoning Province (No. 20092129).

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Affiliations

  1. Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang, People’s Republic of China

    Yongxi Song MD, Feng Zhao MD, Zhenning Wang MD, PhD, Zhuangkai Liu MD, Yeunpo Chiang MD, Yingying Xu MD, PhD, Peng Gao MD & Huimian Xu MD, PhD

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  1. Yongxi Song MD
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  2. Feng Zhao MD
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Correspondence to Zhenning Wang MD, PhD.

Additional information

Yongxi Song and Feng Zhao contributed equally to this work.

Electronic Supplementary Material

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10434_2011_1999_MOESM1_ESM.pdf

Table S1. RT-PCR primers for amplification of miR-194 and the sequences of miR-194 mimics and negative control (PDF 32 kb)

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Song, Y., Zhao, F., Wang, Z. et al. Inverse Association between miR-194 Expression and Tumor Invasion in Gastric Cancer. Ann Surg Oncol 19, 509–517 (2012). https://doi.org/10.1245/s10434-011-1999-2

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  • DOI: https://doi.org/10.1245/s10434-011-1999-2

Keywords

  • Gastric Cancer
  • Gastric Cancer Cell
  • Gastric Cancer Cell Line
  • Gastric Cancer Tissue
  • Noncancerous Tissue