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p21Waf1/Cip1 Polymorphisms and Risk of Esophageal Cancer

  • Translational Research and Biomarkers
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Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

As the main downstream effecter of tumor suppressor p53, p21Waf1/Cip1 functions as a unique link from p53 to cell-cycle arrest and DNA repair. In contrast to p53, p21Waf1/Cip1 has general rare mutations. The natural genetic variants of p21Waf1/Cip1 have thus emerged for study to enhance understanding of interindividual differences in cancer risk. Two polymorphisms in the p21 Waf1/Cip1 gene, i.e., codon 31 in the coding region and IVS2+16 in intron 2, have been identified and appeared to influence the expression of p21Waf1/Cip1. The aim of this study is to investigate the potential association of the above two variants, including one new single-nucleotide polymorphism (SNP) 309 in the promoter region of p21 Waf1/Cip1, with susceptibility to esophageal cancer (EC).

Patients and Methods

The study involved 80 cancer patients and 200 cancer-free controls from Ningxia Region of China. Three variations (codon 31, IVS2+16, and SNP 309) were identified by polymerase chain reaction (PCR) direct sequencing method, and associations of each individual SNP and haplotypes of the three SNPs with esophageal cancer were analyzed.

Results

The correlation results supported that codon 31 Ser homozygosity conferred risk for the process of developing EC [odds ratio (OR) = 2.542, 95% confidence interval (CI) = 1.347–4.730]. In the combined study of the three variations, HapA and HapB appeared to influence the risk of EC.

Conclusions

Our findings indicated that codon 31 Ser allele homozygosity, either alone or in combination with the other two SNPs, may be associated with development of EC. These findings warrant validation in a larger study of EC patients.

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Acknowledgment

The authors would like to thank Dr. Guoshuang Feng for his excellent statistical assistance and advice. This work was supported by National Natural Science Foundation of China (30860320), Ningxia Natural Science Foundation (NZ0880), and projects from Ningxia Educational Ministry (2007298) and Ningxia Medical University (XT200701) to Wenjun Yang.

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Authors and Affiliations

  1. Department of Biotechnology, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China

    Wenjun Yang PhD, Qi Qi MD, Heng Zhang MD & Wenda Xu MD

  2. Key Laboratory of Ningxia Reproduction and Heredity, Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China

    Wenjun Yang PhD, Li Wang, Haiyan Jiao MD & Zhenghao Huo MD

  3. Scientific Technology Research Center of Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China

    Wenjun Yang PhD & Yin Wang PhD

  4. Department of Surgical Oncology, Affiliated Hospital of Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China

    Heng Zhang MD & Wenda Xu MD

  5. Department of Radiology, Affiliated Hospital of Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China

    Zhiqiang Chen MD

  6. Ningxia Hui Autonomous Regional People’s Hospital, Yinchuan, Ningxia, People’s Republic of China

    Xiaowei Dong

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  1. Wenjun Yang PhD
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  2. Qi Qi MD
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  3. Heng Zhang MD
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  4. Wenda Xu MD
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  5. Zhiqiang Chen MD
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  6. Li Wang
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  7. Yin Wang PhD
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  8. Xiaowei Dong
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  9. Haiyan Jiao MD
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  10. Zhenghao Huo MD
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Correspondence to Wenjun Yang PhD or Zhiqiang Chen MD.

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Yang, W., Qi, Q., Zhang, H. et al. p21Waf1/Cip1 Polymorphisms and Risk of Esophageal Cancer. Ann Surg Oncol 17, 1453–1458 (2010). https://doi.org/10.1245/s10434-009-0882-x

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  • DOI: https://doi.org/10.1245/s10434-009-0882-x

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