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PDGF-BB is a Novel Prognostic Factor in Colorectal Cancer

  • Gastrointestinal Oncology
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Human platelet-derived growth factor BB (PDGF-BB) is thought to be involved in human malignancies. Its overexpression has been reported in some human tumors. However, its expression in colorectal cancer has not been studied. We thus investigated the clinicopathological and biological significance of PDGF-BB gene expression in human colorectal cancer.

Experimental Design

Using real-time reverse transcription-PCR, we evaluated PDGF-BB expression status and correlated data with clinicopathological parameters in 60 patients with colorectal cancer. Additionally, we established a colorectal cancer cell line expressing PDGF-BB and investigated its effects on cell invasion and proliferation.

Results

The incidence of vascular invasion was significantly greater in patients expressing PDGF-BB at a high level than in those at a low level (P < .05). Patients with high PDGF-BB expression had a significantly poorer survival rate than those with low PDGF-BB expression (P < .05). A multivariate analysis demonstrated that PDGF-BB expression was an independent prognostic factor. We demonstrated in vitro that cells transduced with PDGF-BB showed greater invasiveness (P < .05) and migration (P < .001) than did mock transduced cells. In a xenograft study, cells transduced with PDGF-BB had higher proliferation rates than mock transfected cells.

Conclusion

PDGF-BB expression may be a new prognostic indicator for patients with colorectal cancer.

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Acknowledgments

We thank Dr. Koichi Tsuji for pathological assistance; we thank Drs. K. Ieta, N. Haraguchi, S. Hirasaki, Y. Kosaka, T. Yokoe, Z. Xiang, T. Ohmachi, K. Mimori, A. Sasaki, and H. Inoue for critical reading of the manuscript; and we also thank Ms. T. Shimooka, Ms. K. Ogata, Ms. M. Oda, Ms. N. Kasagi, and Ms. Y. Nakagawa for their excellent technical assistance. Financial support: This work was supported by the following grant sponsors: CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research, grant numbers 17109013, 17591411, 17591413, 18390367, 18590333, 18659384, and 18790964; The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas, grant No. 18015039; Third Term Comprehensive Ten-Year Strategy for Cancer Control, grant No. 16271201.

Author information

Authors and Affiliations

  1. Department of Surgery, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumibaru, Beppu, 874-0838, Japan

    Yoshito Nakamura MD, PhD, Fumiaki Tanaka MD, PhD, Koshi Mimori MD, PhD, Hiroshi Inoue MD, PhD & Masaki Mori MD, PhD

  2. Department of Pathology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumibaru, Beppu, 874-0838, Japan

    Yasuji Yoshikawa MD, PhD

  3. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8 Honcho Kawaguchi, Saitama, Japan

    Fumiaki Tanaka MD, PhD, Koshi Mimori MD, PhD, Hiroshi Inoue MD, PhD & Masaki Mori MD, PhD

  4. Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo, Japan

    Yoshito Nakamura MD, PhD & Katsuhiko Yanaga MD, PhD

Authors
  1. Yoshito Nakamura MD, PhD
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  2. Fumiaki Tanaka MD, PhD
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  3. Yasuji Yoshikawa MD, PhD
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  4. Koshi Mimori MD, PhD
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  5. Hiroshi Inoue MD, PhD
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  6. Katsuhiko Yanaga MD, PhD
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  7. Masaki Mori MD, PhD
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Corresponding author

Correspondence to Masaki Mori MD, PhD.

Additional information

Grant sponsors: CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research, grant numbers 17109013, 17591411, 17591413, 18390367, 18590333, 18659384, and 18790964; The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas, grant number 18015039; Third Term Comprehensive Ten-year Strategy for Cancer Control, grant number 16271201.

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Nakamura, Y., Tanaka, F., Yoshikawa, Y. et al. PDGF-BB is a Novel Prognostic Factor in Colorectal Cancer. Ann Surg Oncol 15, 2129–2136 (2008). https://doi.org/10.1245/s10434-008-9943-9

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  • DOI: https://doi.org/10.1245/s10434-008-9943-9

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