Tripartite motif-containing 29 (TRIM29) belongs to the TRIM protein family, which has unique structural characteristics, including multiple zinc finger motifs and a leucine zipper motif. TRIM29, also known as ataxia telangiectasia group D complementing gene, possesses radiosensitivity suppressor functions. Although TRIM29 has been reported to be underexpressed in prostate and breast cancer, its expression in gastrointestinal cancer has not been studied.
By use of real-time reverse transcriptase–polymerase chain reaction, we analyzed TRIM29 mRNA expression status with respect to various clinicopathological parameters in 124 patients with gastric cancer. An immunohistochemical study was also conducted.
The expression of TRIM29 was far higher in gastric cancer tumor tissue. Increased TRIM29 mRNA expression was markedly associated with such parameters as histological grade, large tumor size, extent of tumor invasion, and lymph node metastasis. In the TRIM29 high-expression group, it was an independent predictor for lymph node metastasis. Furthermore, patients with high TRIM29 mRNA expression showed a far poorer survival rate than those with low TRIM29 mRNA expression.
TRIM29 expression may serve as a good marker of lymph node metastasis in gastric cancer.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Roder DM. The epidemiology of gastric cancer. Gastric Cancer 2002;5(Suppl 1):5–11
Tominaga S. Epidemiologic trends of stomach cancer in Japan and world. Nippon Rinsho 2001;59(Suppl 4):5–12
Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. Embo J 2001;20:2140–51
Borden KL. RING fingers and B-boxes: zinc-binding protein-protein interaction domains. Biochem Cell Biol 1998;76:351–8
Reddy BA, Etkin LD, Freemont PS. A novel zinc finger coiled-coil domain in a family of nuclear proteins. Trends Biochem Sci 1992;17:344–5
Dho SH, Kwon KS. The Ret finger protein induces apoptosis via its RING finger-B box-coiled-coil motif. J Biol Chem 2003;278:31902–8
Jensen K, Shiels C, Freemont PS. PML protein isoforms and the RBCC/TRIM motif. Oncogene 2001;20:7223–33
Remboutsika E, Lutz Y, Gansmuller A, Vonesch JL, Losson R, Chambon P. The putative nuclear receptor mediator TIF1alpha is tightly associated with euchromatin. J Cell Sci 1999;112:1671–83
Ryan RF, Schultz DC, Ayyanathan K, et al. KAP-1 corepressor protein interacts and colocalizes with heterochromatic and euchromatic HP1 proteins: a potential role for Kruppel-associated box-zinc finger proteins in heterochromatin-mediated gene silencing. Mol Cell Biol 1999;19:4366–78
Tezel G, Nagasaka T, Shimono Y, Takahashi M. Differential expression of RET finger protein in testicular germ cell tumors. Pathol Int 2002;52:623–7
Krutzfeldt M, Ellis M, Weekes DB, et al. Selective ablation of retinoblastoma protein function by the RET finger protein. Mol Cell 2005;18:213–24
Kapp LN, Painter RB, Yu LC, et al. Cloning of a candidate gene for ataxia-telangiectasia group D. Am J Hum Genet 1992;51:45–54
Brzoska PM, Chen H, Zhu Y, et al. The product of the ataxia-telangiectasia group D complementing gene, ATDC, interacts with a protein kinase C substrate and inhibitor. Proc Natl Acad Sci U S A 1995;92:7824–8
Ernst T, Hergenhahn M, Kenzelmann M, et al. Decrease and gain of gene expression are equally discriminatory markers for prostate carcinoma: a gene expression analysis on total and microdissected prostate tissue. Am J Pathol 2002;160:2169–80
Nacht M, Ferguson AT, Zhang W, et al. Combining serial analysis of gene expression and array technologies to identify genes differentially expressed in breast cancer. Cancer Res 1999;59:5464–70
Japanese Research Society for Gastric Cancer. Japanese Classification of Gastric Carcinoma. 13th ed. Tokyo: Kanehara, 1999
Utsunomiya T, Okamoto M, Hashimoto M, et al. A gene-expression signature can quantify the degree of hepatic fibrosis in the rat. J Hepatol 2004;41:399–406
Utsunomiya T, Hara Y, Kataoka A, et al. Cystatin-like metastasis-associated protein mRNA expression in human colorectal cancer is associated with both liver metastasis and patient survival. Clin Cancer Res 2002;8:2591–4
Utsunomiya T, Inoue H, Taguchi K, Shimada M, Sugimachi K, Mori M. G protein gamma 7 expression as a new clinicopathological marker in patients with intrahepatic cholangiocarcinoma. Arch Surg 2002;137:181–5
Masuda TA, Inoue H, Nishida K, et al. Cyclin-dependent kinase 1 gene expression is associated with poor prognosis in gastric carcinoma. Clin Cancer Res 2003;9:5693–8
Ogawa K, Utsunomiya T, Mimori K, et al. Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma. Br J Cancer 2004;91:282–6
Ohmachi T, Tanaka F, Mimori K, Inoue H, Yanaga K, Mori M. Clinical significance of TROP2 expression in colorectal cancer. Clin Cancer Res 2006;12:3057–63
Schlomm T, Luebke AM, Sultmann H, et al. Extraction and processing of high quality RNA from impalpable and macroscopically invisible prostate cancer for microarray gene expression analysis. Int J Oncol 2005;27:713–20
Siewert JR, Bottcher K, Stein HJ, Roder JD. Relevant prognostic factors in gastric cancer: ten-year results of the German Gastric Cancer Study. Ann Surg 1998;228:449–61
Nakamura K, Ueyama T, Yao T, et al. Pathology and prognosis of gastric carcinoma. Findings in 10,000 patients who underwent primary gastrectomy. Cancer 1992;70:1030–7
Itoh H, Oohata Y, Nakamura K, Nagata T, Mibu R, Nakayama F. Complete ten-year postgastrectomy follow-up of early gastric cancer. Am J Surg 1989;158:14–6
Ohta H, Noguchi Y, Takagi K, Nishi M, Kajitani T, Kato Y. Early gastric carcinoma with special reference to macroscopic classification. Cancer 1987;60:1099–106
Sano T, Kobori O, Muto T. Lymph node metastasis from early gastric cancer: endoscopic resection of tumour. Br J Surg 1992;79:241–4
Gotoda T, Yamamoto H, Soetikno RM. Endoscopic submucosal dissection of early gastric cancer. J Gastroenterol 2006;41:929–42
Supported in part by CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (grants 17109013, 17591411, 17591413, 18390367, 18590333, 18659384, and 18790964); and Ministry of Education, Culture, Sports, Science and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas (grant 18015039), third term. We thank T. Shimooka, K. Ogata, M. Oda, N. Kasagi, and Y. Nakagawa for technical assistance.
About this article
Cite this article
Kosaka, Y., Inoue, H., Ohmachi, T. et al. Tripartite Motif-Containing 29 (TRIM29) Is a Novel Marker for Lymph Node Metastasis in Gastric Cancer. Ann Surg Oncol 14, 2543–2549 (2007). https://doi.org/10.1245/s10434-007-9461-1
- Gastric cancer
- Lymph node metastasis