Annals of Surgical Oncology

, Volume 21, Issue 13, pp 4270–4277 | Cite as

The hMLH1 −93G>A Promoter Polymorphism is Associates with Outcomes in Oral Squamous Cell Carcinoma Patients

  • Li-Han Lin
  • Ming-Wei Lin
  • Kwei Mar
  • Chun-Shu Lin
  • Dar-Der Ji
  • Wei-Ping Lee
  • Herng-Sheng Lee
  • Ming-Fang Cheng
  • Kan-Tai Hsia
Head and Neck Oncology

Abstract

Background

The aim of this study was to investigate the impact of hMLH1 polymorphisms on treatment outcomes in patients with oral squamous cell carcinoma (OSCC).

Methods

Genotypings were performed by direct DNA sequencing in peripheral blood leukocytes from 185 male OSCC patients. Patients received primary surgery with or without adjuvant radiotherapy. Two hMLH1 tag single nucleotide polymorphisms (SNPs)—rs1800734 (−93G>A in the promoter) and rs1540354 (in the third intron)—were chosen from the HapMap project. Overall survival (OS) and disease-free survival (DFS) were compared between different genotypes.

Results

The hMLH1 rs1800734 and rs1540354 polymorphisms were in weak linkage disequilibrium (r2 = 0.456). OSCC patients with the rs1800734 AA genotype had a significantly poor prognosis in both OS and DFS. This SNP can also predict the outcomes of OSCC patients with postoperative adjuvant radiotherapy, especially in advanced stage; however, no significant differences in patient outcomes were found for the hMLH1 rs1540354 genotypes.

Conclusions

Our results demonstrate that the hMLH1 −93G>A SNP is found to be associated with patient outcomes in OSCC. This SNP can also predict their treatment outcome of radiotherapy.

Notes

Acknowledgment

This study was supported by grants from the National Science Council NSC 99-2314-B-010-044-MY3, Aim for Top University plan from the Department of Education, Taiwan, and Department of Health, Taipei City Government 96001-62-015.

Conflict of interest

None.

Supplementary material

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Copyright information

© Society of Surgical Oncology 2014

Authors and Affiliations

  • Li-Han Lin
    • 1
  • Ming-Wei Lin
    • 2
  • Kwei Mar
    • 3
  • Chun-Shu Lin
    • 4
  • Dar-Der Ji
    • 5
  • Wei-Ping Lee
    • 6
    • 7
  • Herng-Sheng Lee
    • 8
  • Ming-Fang Cheng
    • 1
    • 8
    • 9
  • Kan-Tai Hsia
    • 1
  1. 1.Institute of Oral Biology, School of DentistryNational Yang-Ming UniversityTaipeiTaiwan
  2. 2.Institute of Public HealthNational Yang-Ming UniversityTaipeiTaiwan
  3. 3.Department of Dentistry, Zhongxiao BranchTaipei City HospitalTaipeiTaiwan
  4. 4.Department of Radiation Oncology, Tri-Service General HospitalNational Defense Medical CenterTaipeiTaiwan
  5. 5.Department of Tropical MedicineNational Yang-Ming UniversityTaipeiTaiwan
  6. 6.Institute of Biochemistry and Molecular BiologyNational Yang-Ming UniversityTaipeiTaiwan
  7. 7.Division of Gastroenterology, Department of MedicineTaipei Veterans General HospitalTaipeiTaiwan
  8. 8.Department of Pathology, Tri-Service General HospitalNational Defense Medical CenterTaipeiTaiwan
  9. 9.Division of Laboratory MedicineHualien Armed Forces General HospitalHualienTaiwan

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