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Salinomycin Selectively Targets ‘CD133+’ Cell Subpopulations and Decreases Malignant Traits in Colorectal Cancer Lines

  • Translational Research and Biomarkers
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

Cancer stem-like cells (CSCs) in colorectal cancers (CRC) may account for the failure of treatments because they are resistant to many current anticancer therapies. Salinomycin, a potassium ionophore, was recently identified as a selective inhibitor of breast CSCs.

Methods

The human CRC cell lines HT29 and SW480 were treated with salinomycin and oxaliplatin. Cell viability was determined with cell counting kit 8. Fraction of CD133+ cell subpopulations was assessed by Flow Cytometric analysis. Clonogenecity and migration were determined with soft agar and Boyden chamber assays. Molecular changes were assessed by immunofluorescence staining, RT-PCR, and Western blot analysis.

Results

We report that salinomycin reduces the proportion of CD133+ subpopulations in human CRC HT29 and SW480 cells. Furthermore, salinomycin treatment decreases colony-forming ability and cell motility in HT29 cells. Moreover, salinomycin downregulates the expression of vimentin and induces the E-cadherin expression in HT29 cells.

Conclusions

This study demonstrates the ability of salinomycin to selectively target “CD133+” cell subpopulations and decrease the malignant traits in colorectal cancer lines.

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Acknowledgment

This study was supported by the National Natural Science Foundation (No. 30901424) and the Leading Medical Talent Foundation of Shanghai Municipality (No. LJ06038). We thank the members of Bing-Ya Liu’s laboratory for helpful comments and discussions, Dr. Zhi-Qiang Chen for reagents, and Xiao-Shuang Yan for technical help.

Disclosure

This study was supported by the National Natural Science Foundation (No. 30901424) and the Leading Medical Talent Foundation of Shanghai Municipality (No. LJ06038). The authors declared no competing financial interests.

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Authors and Affiliations

  1. Department of Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of China

    Tao-Tao Dong, Hou-Min Zhou, Bo Feng, Bo Lv & Min-Hua Zheng

  2. Shanghai Institute of Digestive Surgery, Shanghai, China

    Tao-Tao Dong, Hou-Min Zhou, Bo Feng, Bo Lv & Min-Hua Zheng

  3. Shanghai Minimally Invasive Surgery Center, Shanghai, China

    Tao-Tao Dong, Hou-Min Zhou, Bo Feng, Bo Lv & Min-Hua Zheng

  4. Shanghai Jiaotong University School of Medicine, Shanghai, China

    Lin-Lin Wang

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  1. Tao-Tao Dong
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Correspondence to Min-Hua Zheng.

Additional information

Tao-Tao Dong and Hou-Min Zhou contributed equally to this work.

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Dong, TT., Zhou, HM., Wang, LL. et al. Salinomycin Selectively Targets ‘CD133+’ Cell Subpopulations and Decreases Malignant Traits in Colorectal Cancer Lines. Ann Surg Oncol 18, 1797–1804 (2011). https://doi.org/10.1245/s10434-011-1561-2

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  • DOI: https://doi.org/10.1245/s10434-011-1561-2

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