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Annals of Surgical Oncology

, Volume 17, Issue 12, pp 3120–3128 | Cite as

MicroRNA-203 Expression as a New Prognostic Marker of Pancreatic Adenocarcinoma

  • Naoki Ikenaga
  • Kenoki Ohuchida
  • Kazuhiro Mizumoto
  • Jun Yu
  • Tadashi Kayashima
  • Hiroshi Sakai
  • Hayato Fujita
  • Kohei Nakata
  • Masao Tanaka
Pancreatic Tumors

Abstract

Background

Detection of aberrant microRNA (miR) expression may contribute to diagnosis and prognosis of various cancers. The aim of this study is to evaluate the correlation between miR-203 expression and prognosis of patients with pancreatic adenocarcinoma after curative resection.

Methods

A total of 113 formalin-fixed paraffin-embedded tissue samples of pancreatic adenocarcinoma, 20 samples of chronic pancreatitis, and 8 samples of normal pancreas were obtained. We investigated the association of miR-203 expression measured by quantitative reverse-transcription polymerase chain reaction assays with clinicopathological parameters and survival times.

Results

miR-203 was overexpressed in pancreatic adenocarcinoma samples compared with chronic pancreatitis (P < 0.001) and normal pancreas (P = 0.001) samples. An association between miR-203 expression and clinicopathological factors of pancreatic adenocarcinoma was not observed. On univariate analysis, the high-miR-203 group and the subgroup (20%) of cases with the highest miR-203 overexpression had significantly shorter survival time (P = 0.048 and P = 0.024, respectively). Multivariate analysis revealed that miR-203 expression was an independent predictor of poor prognosis in cases with no residual tumor (relative risk 2.298, P = 0.027).

Conclusions

miR-203 expression is a new prognostic marker in pancreatic adenocarcinoma patients.

Keywords

Chronic Pancreatitis Pancreatic Adenocarcinoma Pancreatic Cancer Cell Line Normal Pancreas FFPE Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and grants from the Takeda Science Foundation, Pancreas Research Foundation of Japan, and Nakajima Foundation.

Supplementary material

10434_2010_1188_MOESM1_ESM.doc (34 kb)
Supplementary material 1 (DOC 33 kb)

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Copyright information

© Society of Surgical Oncology 2010

Authors and Affiliations

  • Naoki Ikenaga
    • 1
  • Kenoki Ohuchida
    • 1
    • 2
  • Kazuhiro Mizumoto
    • 1
    • 3
  • Jun Yu
    • 1
  • Tadashi Kayashima
    • 1
  • Hiroshi Sakai
    • 1
  • Hayato Fujita
    • 1
  • Kohei Nakata
    • 1
  • Masao Tanaka
    • 1
  1. 1.Departments of Surgery and Oncology, Graduate School of Medical SciencesKyushu UniversityFukuokaJapan
  2. 2.Departments of Advanced Medical Initiatives, Graduate School of Medical SciencesKyushu UniversityFukuokaJapan
  3. 3.Kyushu University Hospital Cancer CenterFukuokaJapan

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