Annals of Surgical Oncology

, Volume 14, Issue 12, pp 3403–3411 | Cite as

Cell Cycle Regulators Show Diagnostic and Prognostic Utility for Differentiated Thyroid Cancer

  • Adrienne Melck
  • Hamid Masoudi
  • Obi L. Griffith
  • Ashish Rajput
  • Graeme Wilkins
  • Sam Bugis
  • Steven J. M. Jones
  • Sam M. WisemanEmail author
Endocrine Tumors Original Papers



Differentiated thyroid cancer (DTC) generally has a favorable outcome, but some patients develop local recurrence and/or distant metastases and ultimately die of their disease. Molecular markers that accurately predict tumor behavior are lacking. This study’s aim was to ascertain the role of cell cycle regulators in predicting malignant histology and tumor behavior in DTC.


Tissue microarrays consisting of 100 benign and 105 malignant thyroid lesions, plus 24 lymph node samples, were stained for p16, p21, p27, p53, p57, p63, cyclin D1, cyclin E, and mdm2. Statistical analysis was used to compare the expression of the markers in benign versus DTC lesions and correlate their expression with clinicopathologic characteristics.


p16, p21, cyclin D1, and cyclin E showed significantly (P < .001) increased expression in DTCs compared with benign thyroid lesions (54.7% vs. 5%, 71.7% vs. 38%, 87.1% vs. 45.7%, and 72.3% vs. 37.4%, respectively). There was no significant difference in expression between benign lesions and DTC for the remaining markers. p16 expression correlated significantly with extrathyroidal tumor extension (P = .02) and the presence of cancer in lymph nodes (P = .03). A total of 73% vs. 45% of the cancers of patients with and without lymph node involvement, respectively, stained positive for p16 (P = .01).


There is a statistically significant difference in the expression of p16, p21, cyclin D1, and cyclin E between DTCs and benign thyroid lesions, and p16 expression correlates with clinicopathologic variables predicting poor outcomes for DTC. These results suggest that evaluation of cell cycle derangement in thyroid tumors may serve as a useful tool for both DTC diagnosis and prognosis.


Thyroid cancer Cell cycle regulators Tissue microarray 


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Copyright information

© Society of Surgical Oncology 2007

Authors and Affiliations

  • Adrienne Melck
    • 1
  • Hamid Masoudi
    • 2
    • 3
  • Obi L. Griffith
    • 4
  • Ashish Rajput
    • 3
  • Graeme Wilkins
    • 5
  • Sam Bugis
    • 1
  • Steven J. M. Jones
    • 4
  • Sam M. Wiseman
    • 1
    • 3
    Email author
  1. 1.Department of SurgerySt. Paul’s Hospital, University of British ColumbiaVancouverCanada
  2. 2.Department of PathologySt. Paul’s Hospital, University of British ColumbiaVancouverCanada
  3. 3.Genetic Pathology Evaluation Center at theProstate Research Center of Vancouver General Hospital, British Columbia Cancer Agency, and University of British ColumbiaVancouverCanada
  4. 4.Department of Medical GeneticsUniversity of British Columbia, British Columbia Cancer Agency, and Michael Smith Genome Sciences CenterVancouverCanada
  5. 5.Department of MedicineSt. Paul’s Hospital, University of British ColumbiaVancouverCanada

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