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Topical Application of Vitamin D3-Loaded Hybrid Nanosystem to Offset Imiquimod-Induced Psoriasis

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Abstract

Lipid-polymer hybrid nanoparticles display several benefits over either lipid and/or polymer based systems with respect to enhanced drug loading, good colloidal stability, sustained release profile, and high cellular uptake. The present work rivets on development and evaluation of vitamin D3-loaded monolithic lipid-polymer hybrid nanoparticles (VD3/LPHNPs) for their in vivo anti-psoriatic efficacy. These LPHNPs were prepared using a hot homogenization method and exhibited spherical morphology with a lower particle size (123.1 nm) with narrow PDI (0.234) and efficient encapsulation (76.80%). Further, these LPHNPs demonstrated a sustained release profile of VD3 for up to 3 days following a Korsemeyer-Peppas release model. Further, VD3/LPHNPs were formulated into a topical gel containing 0.005% w/w of VD3. Rheological data suggested that the product exhibited non-newtonian flow properties with characteristic shear-thinning and variable thixotropy features that are desirable for topical formulation. The successful formation of gel structure and its long-term stability were confirmed from the oscillatory studies such as amplitude and frequency sweep tests. In vivo efficacy assessment in imiquimod-induced psoriatic mouse model demonstrated enhanced anti-psoriatic activity of VD3 with improved PASI score when delivered as LPHNPs gel as compared to the free VD3 gel that were further supported by histopathology and immunohistochemistry.

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Acknowledgements

The authors acknowledged Incisive Element LLC, USA, for the financial support.

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Authors and Affiliations

  1. Department of Pharmacy, Birla Institute of Technology and Science, Pilani (BITS, Pilani), Vidya Vihar Campus, Pilani, Rajasthan, 333 031, India

    Sudeep Sudesh Pukale, Anupama Mittal & Deepak Chitkara

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  1. Sudeep Sudesh Pukale
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  3. Deepak Chitkara
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Correspondence to Deepak Chitkara.

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Conflict of Interest

This work was funded by Incisive Element LLC, USA, and might result into commercial products that can be licenced to other pharmaceutical companies or developed in house, in which the authors have a commercial and/or financial interest. The authors (DC and AM) are the founding directors of Nanobrid Innovations Private Limited, India, that is involved in the development of nanotechnology-based products. They have a business and/or financial interest in the operations of the company. The same could be disclosed on request.

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Pukale, S.S., Mittal, A. & Chitkara, D. Topical Application of Vitamin D3-Loaded Hybrid Nanosystem to Offset Imiquimod-Induced Psoriasis. AAPS PharmSciTech 22, 238 (2021). https://doi.org/10.1208/s12249-021-02116-5

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