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Nanostructure of DiR-Loaded Solid Lipid Nanoparticles with Potential Bioimaging Functions

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Abstract

The fluorescence dye-loaded nanoparticles are widely used as bioimaging agents in the field of nanotheranostics. However, the nanoparticles for nanotheranostics usually consist of synthetic materials, such as metal, silica, and organic polymers, which are often biologically incompatible and may arouse toxicity issues. Herein, the potential of near-infrared probe DiR-containing solid lipid nanoparticle suspensions (DiR-SLNS) as the bioimaging agent, which was prepared by lipids and surfactants with excellent biocompatibility, was investigated in this study. The nanostructure of DIR-SLNS system and the distribution of DiR were studied by dissipative particle dynamics (DPD) simulations. The stability of physicochemical properties and fluorescence spectra of DIR-SLNS system were investigated using dynamic laser scattering (DLS), nanoparticle tracking analysis (NTA), and fluorescence spectra. The fluorescence intensity-concentration correlation of DIR-SLNS was also evaluated. As a result, DiR-SLNS demonstrated a “core-shell”-like nanostructure and DiR was mainly distributed in the cetyl palmitate (CP) core rather than the surface of SLNS, which was beneficial to its potential applications in bioimaging. DiR-SLNS exhibited remarkable physicochemical stability as the nanoparticles maintained ~ 90% fluorescence intensity during the 10-day storage time. The correlation between fluorescence intensity and concentration was established and validated using a linear regression model. This study proposed a type of promising candidates in nano-scale with higher safety and fluorescence stability for bioimaging.

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Acknowledgments

We are thankful for the financial support from the National Science Foundation of China (Grant No. 81703431 and 81673375).

Funding

This study received financial support from the National Science Foundation of China (Grant No. 81703431 and 81673375).

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Authors and Affiliations

  1. Guangzhou Novaken Pharmaceutical Co., Ltd., Guangzhou, 510006, People’s Republic of China

    Lei Shu

  2. College of Pharmacy, Jinan University, Guangzhou, 510632, People’s Republic of China

    Fangqin Fu, Zhengwei Huang, Ying Huang & Ping Hu

  3. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, Guangdong, People’s Republic of China

    Xin Pan

Authors
  1. Lei Shu
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  2. Fangqin Fu
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  3. Zhengwei Huang
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  4. Ying Huang
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Contributions

L. Shu performed part of tests and wrote the manuscript. F. Fu analyzed the data and participate in part of experiments. Y. Huang designed the experimental details, check the data and polished the manuscript. P. Hu performed critical reading and helped to improve the language. Z. Huang and X. Pan determined the research framework and supervised the study, manuscript preparation and submission.

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Correspondence to Zhengwei Huang or Xin Pan.

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The authors declare that they have no conflict of interest.

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Lei Shu and Fangqin Fu are regarded as co-first authors.

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Shu, L., Fu, F., Huang, Z. et al. Nanostructure of DiR-Loaded Solid Lipid Nanoparticles with Potential Bioimaging Functions. AAPS PharmSciTech 21, 321 (2020). https://doi.org/10.1208/s12249-020-01847-1

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