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Preparation and Characterization of Honokiol Nanosuspensions and Preliminary Evaluation of Anti-Inflammatory Effect

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Abstract

The study mainly aimed to improve the solubility of honokiol (HK) by preparing honokiol nanosuspensions (HNS). In this study, HNS were obtained using Kolliphor®P407 (P407) as a stabilizer through melting method combined with high pressure homogenization (HPH). The crystalline state of HNS was confirmed through differential scanning calorimetry (DSC) and X-ray Diffraction (XRD). In vitro, the dissolution rate of HNS was significantly improved than that of pure HK. In vivo, higher anti-inflammatory activity was achieved after free HK had been made into HNS. There was no significant difference between the degree of edema (DE) of HNS group and that of aspirin group. Consequently, melting method combined with HPH was a potent technique to prepare HNS. Furthermore, nanosuspension was a valid formulation that could be utilized to enhance the anti-inflammatory effect of HK.

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Funding

This work was financially supported by the National Natural Science Foundation of China (81673830), six talent peaks project of Jiangsu Province (YY053), Major Project and Double first -class innovative team (CPU2018GY28), and National Science and Technology Major Project (2017zx09101001005).

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Authors and Affiliations

  1. Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210028, China

    Xinyue Lu & Zhenhai Zhang

  2. Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China

    Xinyue Lu & Zhenhai Zhang

  3. Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 211198, China

    Xinyue Lu, Xiaoyu Lu & Huixia Lv

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  1. Xinyue Lu
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  2. Xiaoyu Lu
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  4. Huixia Lv
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Correspondence to Zhenhai Zhang or Huixia Lv.

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Lu, X., Lu, X., Zhang, Z. et al. Preparation and Characterization of Honokiol Nanosuspensions and Preliminary Evaluation of Anti-Inflammatory Effect. AAPS PharmSciTech 21, 62 (2020). https://doi.org/10.1208/s12249-019-1602-x

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