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A Simple and Rapid Method for Deposition and Measurement of Drug Transport Across Air Interface Respiratory Epithelia

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Abstract

The purpose of this study was to present a novel and simple drug deposition method to evaluate drug transport of aerosol microparticles across airway epithelial cells. Microparticles containing ciprofloxacin HCl (Cip) and doxycycline (Dox), alone or in a 50:50% w/w ratio, were spray dried and suspended using 2H, 3H-perfluoropentane, model propellant. The suspension was then used to assess deposition, and transport of these drug microparticles across sub-bronchial epithelial Calu-3 cells was also studied. In comparison with other methods of depositing microparticles, this proposed method, using drug suspended in HPFP, provides control over the amount of drugs applied on the surface of the cells. Therefore, cell permeability studies could be conducted with considerably smaller and more reproducible doses, without the physicochemical characteristics of the drugs being compromised or the use of modified pharmacopeia impactors. The suspension of microparticles in HPFP as presented in this study has provided a non-toxic, simple, and reproducible novel method to deliver and study the permeability of specific quantity of drugs across respiratory epithelial cells in vitro.

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Author notes

  1. Mehra Haghi

    Present address: Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia

Authors and Affiliations

  1. Respiratory Technology, Woolcock Institute of Medical Research, 431 Glebe Point Road, Sydney, Australia

    Mehra Haghi, Paul M Young & Daniela Traini

  2. Discipline of Pharmacology, Faculty of Medicine and Health, University of Sydney, Sydney, Australia

    Paul M Young & Daniela Traini

Authors
  1. Mehra Haghi
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  2. Paul M Young
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  3. Daniela Traini
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Correspondence to Daniela Traini.

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Haghi, M., Young, P.M. & Traini, D. A Simple and Rapid Method for Deposition and Measurement of Drug Transport Across Air Interface Respiratory Epithelia. AAPS PharmSciTech 19, 3272–3276 (2018). https://doi.org/10.1208/s12249-018-1170-5

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  • DOI: https://doi.org/10.1208/s12249-018-1170-5

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